Back close

Course Detail

Course Name Molecular methods lab
Course Code 24MMD281
Program B. Sc. Honors (with Research) in Molecular Medicine
Semester 3
Credits 2
Campus Kochi

Syllabus

Experiment
  1. Preparation of media and antibiotics for bacterial culture
  2. Isolation of plasmid DNA from bacteria
  3. RAPD PCR
  4. Agarose gel electrophoresis
  5. Preparation of competent cells
  6. Bacterial Transformation methods.
  7. Screening of the clones.

Objectives and Outcomes

Course Outcome

CO1 Can prepare stock solutions and media components for bacterial culture

CO2 Can explain the principles behind the plasmid DNA isolation methods, RAPD PCR, and agarose gel electrophoresis.

CO3 Can isolate plasmid DNA, prepare competent cells, perform transformation experiments and screen for positive clones.

CO4 Can follow general safety routines for laboratory work in molecular biology.

CO5 Can plan experimental work based on a protocol and critically evaluate and discuss experimental results.

Program Outcome

PO1: Bioscience Knowledge

PO2: Problem Analysis

PO3: Design/Development of Solutions

PO4: Conduct Investigations of complex problems

PO5: Modern tools usage

PO6: Bioscientist and Society

PO7: Environment and Sustainability

PO8: Ethics

PO9: Individual & Team work

PO10: Communication

PO11: Project management & Finance

PO12: Lifelong learning

c

C O

PO1 PO2 PO3 PO4 PO5 PO6 PO7 PO8 PO9 PO10 PO11 PO12
CO 1 3 1 1 3 1
CO 2 3 3 3 3 1 1 3 1
CO 3 3 2 3 3 3 1 3 1
CO 4 3 1 1 1
CO 5 3 3 2 2 2 1

 

Program Specific Outcomes. (PSO)

PSO 1 – Chemical and physical basis of biology

PSO 2 – Computational science in biology and medicine

PSO 3 – Biochemical and physiological complexity in biology and medicine

PSO 4 – Molecular technology in biology and medicine

PSO 5 – Cell based approaches in diagnosis and therapy

PSO 6 – Microorganisms in medicine

PSO 7 – Nanoscale entities and its significance in medicine

PSO 8 – Tissue architecture engineering in medicine

PSO 9 – Compounds as drugs and its efficacy

PSO 10 – Bioinformatics and artificial intelligence in medicine

PSO 11 –Technology in personalizing medicine

PSO 12 – Protein structural complexity in medicine

PSO 13 – Projecting science and medicine to public

C O

PSO1 PSO2 PSO3 PSO4 PSO5 PSO6 PSO7 PSO8 PSO9 PSO10 PSO11 PSO12 PSO13
CO 1 1 1 2 1 1
CO 2 2 3 2 1 1
CO 3 1 2 3 1 1 1
CO 4 1 1 1
CO 5 1 1 1

Evaluation Pattern

Internal Assessment – 30% 

Records 

Evaluation 

30% 

   

30%

End Semester Examination- 50% 

Practical

Exam 

60% 

Assessment

Viva 

10%

   

70%

 

Total

100%

DISCLAIMER: The appearance of external links on this web site does not constitute endorsement by the School of Biotechnology/Amrita Vishwa Vidyapeetham or the information, products or services contained therein. For other than authorized activities, the Amrita Vishwa Vidyapeetham does not exercise any editorial control over the information you may find at these locations. These links are provided consistent with the stated purpose of this web site.

Admissions Apply Now