Experiment
- Preparation of media and antibiotics for bacterial culture
- Isolation of plasmid DNA from bacteria
- RAPD PCR
- Agarose gel electrophoresis
- Preparation of competent cells
- Bacterial Transformation methods.
- Screening of the clones.
Course Name | Molecular methods lab |
Course Code | 24MMD281 |
Program | B. Sc. Honors (with Research) in Molecular Medicine |
Semester | 3 |
Credits | 2 |
Campus | Kochi |
Course Outcome
CO1 Can prepare stock solutions and media components for bacterial culture
CO2 Can explain the principles behind the plasmid DNA isolation methods, RAPD PCR, and agarose gel electrophoresis.
CO3 Can isolate plasmid DNA, prepare competent cells, perform transformation experiments and screen for positive clones.
CO4 Can follow general safety routines for laboratory work in molecular biology.
CO5 Can plan experimental work based on a protocol and critically evaluate and discuss experimental results.
Program Outcome
PO1: Bioscience Knowledge
PO2: Problem Analysis
PO3: Design/Development of Solutions
PO4: Conduct Investigations of complex problems
PO5: Modern tools usage
PO6: Bioscientist and Society
PO7: Environment and Sustainability
PO8: Ethics
PO9: Individual & Team work
PO10: Communication
PO11: Project management & Finance
PO12: Lifelong learning
c
C O |
PO1 | PO2 | PO3 | PO4 | PO5 | PO6 | PO7 | PO8 | PO9 | PO10 | PO11 | PO12 |
CO 1 | 3 | – | – | – | 1 | 1 | – | – | 3 | – | – | 1 |
CO 2 | 3 | 3 | 3 | 3 | 1 | 1 | – | – | 3 | – | – | 1 |
CO 3 | 3 | 2 | 3 | 3 | 3 | 1 | – | – | 3 | – | – | 1 |
CO 4 | 3 | 1 | – | – | – | 1 | – | – | – | – | – | 1 |
CO 5 | 3 | 3 | 2 | 2 | – | 2 | – | – | – | – | – | 1 |
Program Specific Outcomes. (PSO)
PSO 1 – Chemical and physical basis of biology
PSO 2 – Computational science in biology and medicine
PSO 3 – Biochemical and physiological complexity in biology and medicine
PSO 4 – Molecular technology in biology and medicine
PSO 5 – Cell based approaches in diagnosis and therapy
PSO 6 – Microorganisms in medicine
PSO 7 – Nanoscale entities and its significance in medicine
PSO 8 – Tissue architecture engineering in medicine
PSO 9 – Compounds as drugs and its efficacy
PSO 10 – Bioinformatics and artificial intelligence in medicine
PSO 11 –Technology in personalizing medicine
PSO 12 – Protein structural complexity in medicine
PSO 13 – Projecting science and medicine to public
–
C O |
PSO1 | PSO2 | PSO3 | PSO4 | PSO5 | PSO6 | PSO7 | PSO8 | PSO9 | PSO10 | PSO11 | PSO12 | PSO13 |
CO 1 | 1 | – | 1 | 2 | – | 1 | – | – | – | – | 1 | – | – |
CO 2 | 2 | – | 3 | 2 | – | 1 | – | – | – | – | 1 | – | – |
CO 3 | 1 | – | 2 | 3 | – | 1 | – | – | – | – | 1 | – | 1 |
CO 4 | – | – | 1 | 1 | – | – | – | – | – | – | – | – | 1 |
CO 5 | – | – | 1 | 1 | – | – | – | – | – | – | – | – | 1 |
Internal Assessment – 30% |
||
Records |
Evaluation |
30% |
30% |
||
End Semester Examination- 50% |
||
Practical |
Exam |
60% |
Assessment |
Viva |
10% |
70% |
||
Total |
100% |
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