COURSE SUMMARY
Course Title: 
Mass Spectrometry & Proteomics
Course Code: 
BIO527
Year Taught: 
2019
Semester: 
3
Degree: 
Postgraduate (PG)
School: 
School of Biotechnology
Campus: 
Amritapuri

'Mass Spectrometry & Proteomics' is a course offered for the MSc. Biotechnology and MSc. Bioinformatics at School of Biotechnology, Amrita Vishwa Vidyapeetham.

History of mass spectrometry; Basics of MS instrumentation; Ionization sources-Electron Impact (EI), Chemical ionization (CI), Fast Atom Bombardment (FAB), Electrospray (ESI), Matrix Assisted Laser Desorption and Ionization (MALDI); Mass analyzers- Quadrupole, Traps (Linear trap, 3D trap, Orbitrap, Ion Cyclotron Resonance), Time-of Flight, Hybrid analyzers; Detectors- Electron multiplier, Microchannel plate; Ion mobility mass spectrometry. 

Fundamental parameters of mass spectrometers- Mass accuracy, Resolution, Sensitivity;  Tandem mass spectrometry; Fragmentation techniques- Collision induced dissociation (CID), Electron transfer dissociation (ETD), Electron Capture Dissociation (ECD), Post source decay (PSD), Selected Reaction Monitoring (SRM), Multiple Reaction Monitoring (MRM); MS data interpretation of biomolecules. 

History of proteomics; Protein and peptide separation techniques- Electrophoresis, HPLC; Protein identification- Peptide mass finger printing, Data dependent MS/MS, Data independent MS/MS; Database search engines- Mascot, Sequest; Proteomic work-flows- In-gel digestion, In-solution digestion; Protein validation; Top-down proteomics. 

Posttranslational modification analysis- Phosphorylation, Glycosylation, Disulfide bond determination. 

Quantitative proteomics- Stable isotope labeled quantitation (ICAT, iTRAQ, SILAC, TMT), Label-free quantitation (emPAI, Spectral count, Ion intensity).