Qualification: 
M. Pharm.
asha19484@aims.amrita.edu

Ms. Asha Asokan Manakkadan joined Amrita School of Pharmacy in July 2013. She completed her Bachelor's Degree in Pharmacy from Grace College of Pharmacy, Calicut University and her Master's Degree in Pharmaceutical Chemistry from Amrita School of Pharmacy. After obtaining her post graduation, she started her career in teaching in the year 2012. She worked for eight months in Lisie College of Pharmacy, Lisie Medical & Educational Institutions, Kochi as lecturer. Then she joined Amrita School of Pharmacy from July 2013 onwards. She has 5 years of teaching and research experience and has several publications in national and international journals. Ms. Asha's current research focuses on Insilico drug design, synthesis of pharmacologically important compounds, Medicinal chemistry drug design, Phytochemical screening. She is presently supervising M. Pharm. and B. Pharm. students in their research project on Computational Drug design and Medicinal Chemistry.

Publications

Publication Type: Journal Article

Year of Publication Publication Type Title

2017

Journal Article

S. .T.S, ,, Chittethu, A. B., Jose, A., Balasubramanian, R., and Asha Asokan Manakadan, “Utility of Isatin Semicarbazones in Mammary Carcinoma Cells - A Proof of Concept Study”, Journal of Young Pharmacists, 2017.[Abstract]


The present study was aimed to determine cytotoxic and apoptotic activity of isatin semicarbazones against mammary carcinoma. Methodology: The preparation of these target compounds involved substitution at the third position of the isatin nucleus along with chemical modification at the nitrogen atom of the scaffold to obtain more potent derivatives. The synthesized analogs were characterized by recording their melting point and Rf values along with IR, NMR and mass spectra. An antioxidant study and Cytotoxic Studies were performed by DPPH, Nitric oxide scavenging method and MTT assay methods. Results: The results revealed that all the test compounds (5a-5d) exhibited good anti oxidant activity. The synthesized compounds were then screened for in-vitro cytotoxicity by MTT cell proliferation assay method against breast cancer cell lines including MCF-7 and BT-549 with derivative 5a exhibiting maximum activity in both the cell lines. DNA fragmentation studies further indicated that 5a was involved in apoptotic process. Conclusion: Interestingly, it was observed that while all the synthesized compounds seemed active, 5a exhibited a superior ability to induce apoptosis. More »»

2017

Journal Article

A. Rajendran, Martin, S., Eso, R., Asha Asokan Manakadan, and .T.S, S., “Computational simulation studies of various flavonoid subclasses on treating non-small cell lung carcinoma associated with cigarette smoking”, Journal of Pharmaceutical Sciences and Research, vol. 9, pp. 1117-1121, 2017.[Abstract]


Lung cancer is one of the most prevailing cancers that cause death worldwide. Non-small cell lung cancer is getting tremendously increased day-by-day because of cigarette smoking. On exposure to the tobacco carcinogen- nicotine derived nitrosamine ketone (NNK) leads to the over expression of hepatocyte growth factor (HGF) and c-Met protein,that triggers processes such as metastasis, angiogenesis, anti-apoptosis, enhanced cell growth and motility. Methotrexate treats NSCLC; with major side effects leucopenia and inflammation. So it was essential to propose a novel drug having more potential, least side effects and better therapeutic efficacy. The uptake of flavonoid foods can reduce the risk and further progression of lung cancer. Apart from antitumor activity, flavonoids also possess blood cell production and anti-inflammatory actions. Hence c-Met protein resides attractive binding sites for the invention of new drugs for NSCLC by the incorporation of computational simulation tools and softwares. The docking analysis was performed using Arguslab for the identification of best ligands by predicting the ligand conformation in the protein active sites and assessment of binding affinities. Among 28 ligands were docked with the protein (pdb id: 5EOB) all had shown higher docking scores than the standard drug methotrexate. Thus, we can conclude that flavonoids can become a promising lead in designing a new and improved drug target that are beneficial in NSCLC therapy. Further analysis can be conducted for the synthesis of these drug targets to determine its actions in both in vivo and in vitro studies. © 2017, Pharmainfo Publications. All rights reserved.

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2017

Journal Article

T. James, Rajendran, A., Martin, S., Easo, R., Krishnan, N. C., Asha Asokan Manakadan, and .T.S, S., “Computational Approach on The Drug Affinity Studies of Substituted (4-Aminophenyl)Benzothiazole Derivatives Against Colon Cancer”, Research Journal of Pharmaceutical, Biological and Chemical Sciences (RJPBCS), vol. 8(2), pp. 402-407, 2017.[Abstract]


Colon cancer is the growth arising from the inner wall of colon, which is a part of large intestine. Wnt-signalling pathway has been recognized as a crucial factor in carcinogenesis. Mutations in this pathway lead to the accumulation of beta –catenin which initiates oncogenesis. Thus beta catenin is selected as the target to resist against the uncontrolled growth. In this work, a total of 10 substituted (4-aminophenyl) benzothiazole derivatives were selected for docking studies. These molecules were selected based on their potent antitumor, antifungal, antimicrobial, anthelmintic antidiabetic, and anticonvulsant, anti-inflammatory and antimalarial properties. The primary and secondary characterizations of protein(PDB ID:3SLA) were achieved from pdb structures using the aid of protparam and sopma tools.Insilico docking analysis were carried out, using Argus lab version 4.0 based on their scoring functions and Lipinski rule of 5 .It revealed that these derivatives have shown better docking score than the standard drug RALTITREXED; hence, can be used to reduce one of its side effect inflammation , and showed strong anticancer activity by the suppression of beta catenin, thus making them possible inhibitors against colon carcinogenesis. The main objective of our research work is to conclude that substituted (4-aminophenyl) benzothiazole derivatives are better drugs than RALTITREXED as it shows higher binding energy with the modeled protein. So these substituted derivatives can be used as suitable drug of choice against colon cancer.

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2016

Journal Article

V. Vishnu, .T.S, S., and Asha Asokan Manakadan, “In silico strategy for designing of novel chromene and indole derivatives to combat diabetes mellitus”, Research Journal of Pharmaceutical, Biological and Chemical Sciences, vol. 7, pp. 1098-1105, 2016.[Abstract]


Diabetes Mellitus is an assorted group of metabolic disorders due to the inability of pancreas to secrete insulin or the insensitivity of the cells to insulin. This work has been chosen considering the vital role of aldose reductase inhibitors in Diabetes Mellitus through the polyol pathway. Consequently an effective cont rol is the key to tackle this abnormal condition. In silico analysis for the development of aldose reductase inhibitors were carried out using both phytochemicals and generation of new lead molecules as ligands. The phytochemicals selected for the study were Allicin, Galegine, Rhamnoside, Quercetin, Trigonelline and Ferulic Acid. The lead molecule nucleus finalized were that of chromenes and indole derivatives. The present study included the analysis of parameters relating to proteins such as primary and s econdary structure analysis, subcellular location, analysis of cavities, ADME as well as docking results. Positive results were shown by Allicin, Galegine, Rhamnoside, Quercetin, Trigonelline and Ferulic Acid when compared with the standard drugs like Epalrest and Sorbinil. The lead molecules of chromeme derivative AR2,methyl2,2-dimethyl3-(4oxo-3-phenoxymethyl)-4H-chromrn-8-yl)propanoate and indole derivative AR7, methylidene(([1-(phenoxymethyl)-2,3-dihydro-1H-indol-5yl]oxy))amine screened displayed significant results over the phytochemicals and the standard drugs used. Further in vitro pharmacological activities might prove to be useful to substantiate the results.

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2016

Journal Article

A. S. Sekhar, .T.S, S., Baskar, V., and Asha Asokan Manakadan, “In Silico Approach of Apoptosis Inducing Ability of Different Indole Derivatives by Interaction with CASPASE9”, Int. J. Pharm. Sci. Rev. , vol. 40, no. 2, pp. 92-102 , 2016.[Abstract]


Indole compounds are well known for their wide variety of pharmacological activities. It is the combination of benzene with pyrrole
ring. Compounds having indole group are biologically important. They are used as antimicrobial, antiviral, antituberular, antiinflammatory,
anticancer, ant-diabetic and anticonvulsant agents. Because of the wide variety of biological application, several
substituted indole derivatives are studied for their molecular structure, molecular docking and bioavailability. The purpose of the
study is to carry out the docking studies of indole derivatives containing electrophilic substitution and nucleophilic substitution with
the anticancer target casp9. Fifty compounds were designed and by using Argus lab version 4.0.1. Their docking score was calculated
and compared with the standard drug casodex. The drug likeness of compounds was performed using Lipinski rule of five. Result
showed that 1,1’-(1H-indole-5,6-diyl)diethanone and 5-(trichloromethyl)-1H-indole and the phytoconstituent curcumin showed
better docking score than that of the standard drug casodex. It is concluded that certain indole derivatives show greater affinity with
casp9 protein. These compounds may be helpful in studying anticancer targets for casp9 protein.

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2016

Journal Article

N. Prasad Nair, Joy, J., Kumar, S. S., .Sathianarayanan, S., Asha Asokan Manakadan, and .T.S, S., “In-silico Docking Studies of Coumarin Derivatives as Caspase 8 and PDE4 Antagonist.”, Research Journal of Pharmacy and Technology , 2016.[Abstract]


Coumarin, a well-known organic compound for its wide range of activities, incorporating a benzene and pyrone ring together, belongs to benzopyrone family. Piroxicam is the drug that is used in the treatment of inflammation as well as cancer but it possess certain side effects like constipation, blurred vision etc. Coumarin shows anti-inflammatory and anticancer activity based on different substitution on it. The purpose of this study is to screen the best target among Caspase-8 and PDE4. Arguslab 4.0.1 docking analysis was performed to find out the best ligand which shows highest score for anti-inflammatory and anti-cancer activity and compared with the standard drug piroxicam. The phytoconstituents like isofraxidin and scopoletin having coumarin pharmacophore were also used for docking analysis. Among the twenty eight analogues of coumarin, twenty four showed good score for caspase 8 and all compounds possess good score for PDE4 compared to piroxicam. The best target among caspase 8 and PDE4 for anti- inflammatory and anti-cancer activity was found to be PDE4 and ethyl substitution at the 7th position of coumarin derivatives showed good affinity against PDE4.

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2015

Journal Article

Asha Asokan Manakadan, .T.S, S., S, S. K., and J, C., “A Computational In silico Approach for Identification of Indian Herbs for the Treatment of Alzheimer's Disease”, Research Journal of Pharmaceutical, Biological and Chemical Sciences, vol. 6, no. 1, pp. 414-422, 2015.[Abstract]


Alzheimer's disease (AD) is a neuronal disorder associated with dementia and EphB2 gene play a crucial role in the NMDA signaling pathway helping to restore cognitive functions. Therefore, attempt has been made to study the pharmacogenomics of the gene to identify the individual variations by the functional effect of Single Nucleotide Polymorphisms (SNPs). Out of the 4997 SNPs, 9 SNPs were found to be damaging in nature with deleterious amino acid substitutions. Computer Aided Drug Designing (CADD) was instrumental to find out the effective constituent among the plant sources, Withania somnifera, Centella asiatica, Catharanthus roseus, Curcuma longa and Bacopa monnieri with Anti-Alzheimer Activity. The corresponding 21 plant constituents analyzed were Withanolide E, Withanolide A, Withanoside IV, Withaferine A, Anaferine, Beta-Sitosterol, Quercetin, Asiatic acid, Asiaticoside, Campesterol, Madecassoside, Madecassic acid, Vincristine, Vincamine, Vinblastine, Catharanthine, Kaempferol, Curcumin, Ascorbic acid, Linalool and Bacoside A. Molecular property, bioactivity parameter as well as Protein-Ligand Dynamic interaction were analyzed to determine the drug likeness of the selected constituents. Bench marking were done using an antioxidant Butylated hydroxytoluene (BHT). The in silico analysis performed indicate the selection of natural compounds like Withanoside IV, Asiaticoside, Madecassoside, Vincristine, Vinblastine and Bacoside A to show significant binding interaction with 1B4F protein.

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2015

Journal Article

S. G. Gayathri, Vishnu, V., Shibu, S., .T.S, S., and Asha Asokan Manakadan, “Computational identification and analysis of phytoconstituents inhibiting vital proteins in Mycobacterium tuberculosis”, Journal of Chemical and Pharmaceutical Research, vol. 7, no. 11, pp. 170-176, 2015.[Abstract]


Tuberculosis caused by Mycobacterium tuberculosis is disproportionately one of the major burden in health care system. In the present work we have extensively studied the target proteins Pantothenate synthetase, Thymidylate kinase and Filamentous temperature sensitive protein Z (FtsZ) for its significance in the treatment for tuberculosis. Computer aided drug design (CADD) aided to predict the most effective phytoconstituents for the tre3atment for tuberculosis among the 28 plant sources used for the study. The phytoconstituents taken up for the investigation included Agnuside, Alloin, Alliin, Alpha-amyrin, Alizarin, Aloe-emodin, Berberine, Coumaric acid, Kaempherol, Kumatakenin, Luteolin, Mimosine, Mangiferin, Myricetin, Niacin, Oleanane, Orientin, Protocatechuic acid, Piperine, Quinoline, Rottlerin, Sesamin, Scopoletin, Serotonin, Taraxerol, Thiamine, Ursolic acid and Xanthone. The computational approach included the prediction of Molecular properties, bioactive scores, the analysis of primary and secondary structures of proteins and the binding interaction calculation using docking principles. The results of in silico study pointed out that the phytoconstituents such as Alizarin, Kaempherol, Myricetin, Alliin, Coumaric acid, Protocatechuic acid, Aloin, Agnuside, Aloe-emodin, Mangiferin and Rottlerin elicits good interaction by inhibiting the selected proteins as compared with the standard drug Isoniazid. Further investigation is essential for the development of potent inhibitors for the control of the disease.

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2015

Journal Article

Dr. S. Sathianarayanan, S, S. M., Kumar, R. S., V, S., Menon, S. V., Mohan, S., .T.S, S., Asha Asokan Manakadan, and Suja, S., “A Computational approach for identification of Phytochemicals for targeting and optimizing the inhibitors of Heat shock proteins”, Research Journal of Pharmacy and Technology., vol. 8, no. 9, pp. 1199- 1204, 2015.[Abstract]


Pancreatic cancer is a class of disease characterized by uncontrolled cell growth that begins in the pancreas. Heat shock proteins (Hsps) are chaperone proteins that on inhibition can prove to be effective in pancreatic cancer treatment. In cancer cells, Hsp90 significantly displays a proliferative potential of the malignant cells, so the inhibition of the protein can prove to be valuable to combat pancreatic cancer. Computer Aided Drug Designing (CADD) was instrumental to find out the effective constituent among the 35 plant sources selected. The Phytochemicals used for the present study were Curcumin, Allicin, Pinene, Tetrahydrocannabinol, Astragalin, Arginine, Scopoletin, Baicalin, Stylopine, Carvacrol, Quercetin, Thymol, Guaiaretic acid, Coumarin, Chlorogenic acid, Taraxacin, Ascorbic acid, Protocatechuic acid, Withaferin A, Triptolide, Ursolic acid, Cucurbitane, Thymoquinone, D-Carvone, Eugenol, Ellagic acid, Lapachol, Genistein, Emodin, Chelidonine, Caffeine, Catechin, Geraniol, Myrcene and Niacin. The preliminary studies such as the Primary and Secondary structure analysis of the protein were carried out. Molecular property based on Lipinski rule of 5, bioactivity parameters as well as Protein-Ligand Dynamic interaction were analyzed to determine the drug likeness of the ligands with the protein 4L94. The results of the in silico docking analysis indicate the selection of natural compounds such as Cucurbitane, Myrcene and Pinene as they elicited significant binding interaction in inhibiting Heat shock proteins for targeting pancreatic cancer which was comparable with that of the standard drug Gemcitabine. Further in vivo studies may be carried out to prove the same.

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2015

Journal Article

R. Thomas, Hari, R., Joy, J., Krishnan, S., A.N, S., Nair, S. S., Asha Asokan Manakadan, ,, and .T.S, S., “In silico Docking Approach of Coumarin Derivatives as an Aromatase Antagonist”, Research Journal of Pharmacy and Technology , vol. 8, no. 12, pp. 1673-1678 , 2015.[Abstract]


<p>Coumarins are pharmacologically active moiety well known for their wide variety of activities. Coumarin is the combination of benzene and pyrone ring and it belongs to benzo pyrone family. It is available from plants, microorganisms etc. Depending on various substitutions on different positions of coumarin, we get different pharmacological properties like anticancer, antioxidant, antifungal, anti-inflammatory, antiviral, anticoagulant etc. The purpose of this study is to carry out the docking studies of coumarin derivatives containing electrophilic and nucleophilic substitutions with the anticancer target aromatase by using Arguslab version 4.0.1. Fifty four lead molecules were designed and their docking scores were compared with the standard drug Gefitinib. The validation of ligands were performed using Lipinski rule of five. It was observed that nineteen ligands showed better docking score than the standard drug Gefitinib. Phytoconstituents like Isofraxidin, Scopoletin and umbelliferone from Compositae family, which were the source of coumarin nucleus were also taken for the docking analysis. It was observed that most of the lead molecules showed higher docking score than the phytoconstituents.</p>

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2014

Journal Article

Asha Asokan Manakadan, .T.S, S., and , “In silico analysis of functional snps of Alox12 gene and identification of pharmacologically significant flavonoids as lipoxygenase inhibitors”, International Research Journal of Pharmacy , vol. 5, no. 6, pp. 502-507, 2014.[Abstract]


Cancer is a disease affecting any part of the body and in comparison with normal cells there is an elevated level of lipoxygenase enzyme in different cancer cells.

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2010

Journal Article

Dr. Aneesh T. P., Belzik, N., Nisha, A. R., Resiya, S., Silvipriya, K. S., Asha Asokan Manakadan, and Sonu, J., “A tool in chemical synthesis: Combinatorial library design”, Inventi Impact: Med Chem, 2010.[Abstract]


Combinatorial synthesis revolutionized the drug industries in this century. After a sequence of procedures parallel generation of molecules carried out and follows high throughput screening assays. Genomic and proteomic studies lead us to design promising molecule that bind in diseased targets. Success in lead discovery requires computational methods, data mining tools and docking procedures. Automated computational technologies reduced time and wastage of money in synthesizing new molecules while whole world is suffering from worst diseases. Building of novel drug is a complex process. In olden days active compounds used in drug discovery programs have been natural products, isolated from plant, animal or fermentation sources. Combinatorial chemistry is one of the important technique developed by researchers in the pharmaceutical industry to minimize the time and costs associated with producing effective and competitive new drugs. By combinatorial chemistry large number of analogues is generated using the same reaction conditions, the same reaction vessels. Traditional synthesis of drug was tedious process which involves one compound at a time

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2010

Journal Article

S. Subramanian, Sankar, V., Asha Asokan Manakadan, Ismail, S., and Andhuvan, G., “Formulation and evaluation of Cetirizine dihydrochloride orodispersible tablet”, Pakistan Journal of Pharmaceutical Sciences, vol. 23, no. 2, pp. 232-235, 2010.[Abstract]


Cetirizine orodispersible tablets were prepared to achieve quick onset of action and for maximum bioavailability. Tablets were prepared using cetirizine along with camphor and mannitol in the proportion of 1:1:1, 1:1:3, and 1:1:6. The flow property of granules was found to be good for the formulation CZ2 (1:1:3). The hardness and friability of all the formulations were found to be within the standard limit for orodispersible tablets. Disintegration time was found to be rapid in formulation CZ2 (1:1:3).The in vitro dissolution time was found to be 100% in 11 minutes for the formulation CZ2 (1:1:3).

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2010

Journal Article

J. K. varghese, sukhumaran, T. P. Mamatha, Asha Asokan Manakadan, and Syama, S., “Phytochemical Investigation of Seaweed Ulva Reticulata From the Coast of Bakel, Kasaragod of Kerala State In India”, International Journal of Pharma World Research, vol. 1, no. 2, 2010.

2010

Journal Article

J. K. varghese, Josemon, T., Nagalekshmi, R., Joshy, B., Asha Asokan Manakadan, Jason, C. K., and Rohith., V. A., “Physicochemical Studies of Some Species of Mangroves in Kochi of Kerala State in India”, Inventi Rapid Nutraceuticals, vol. 1, no. 2, 2010.[Abstract]


Mangroves are a special group of vascular plants that occur in saline coastal habitats. Some mangroves are utilized for a wide range of conditions including bacterial, fungal and viral diseases. These specialized plants are able to tolerate extreme environmental conditions. The economical advantages of products from mangrove ecosystems are many and beneficial. Many of the mangrove species are a rich source of various important and unique phytoconstituents. The mangroves provide food and wide variety of traditional products. Keeping in view the high medicinal value and edible property, the physico-chemical studies of the some of the species of mangroves in Kochi of Kerala state in India such as Acanthus ilicifolius and Sonneratia caseolaris has been carried out. The successive extraction of the plant material was done with different solvents. Physico chemical parameters applied for the present study include ash values, extractive values, moisture content, TLC and fluorescent analysis. Thin layer chromatography has been carried out for various phytochemical constituents and the inference is noted. The findings of physico chemical analysis will be useful for establishing the standards on the purity and quality of the plant, which will be relevant in the phytomedicine research.

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Publication Type: Conference Proceedings

Year of Publication Publication Type Title

2014

Conference Proceedings

P. K. Krishnan Namboori, Asha Asokan Manakadan, S, K. A., Saranya, T. S., .S.Silvipriya, K., and .S.Nimmi, O., “Insilico strategy for designing of novel molecules to combat diabetes mellitus with focus on aldose reductase inhibitors”, International conference on emerging trends in drug discovery-AICADD 2014. 2014.

2013

Conference Proceedings

N. P. K, M, D. O., N, B., KS, S., A, J., Asha Asokan Manakadan, and P, R. U., “Computational modeling of Environmentally Responsive Hydrogels (ERH) for drug delivery system”, Current Computer Aided Drug Design, vol. 9. pp. 76-82, 2013.[Abstract]


The present work aims at computational analysis of environmentally responsive hydrogels with enormous prospective in the formulation aspect of drug delivery systems. The drug delivery potential of hydrogels to the targets is owing to the specific stimuli responsive nature of the hydrogels. The environmental factors looked upon in the study are changes in pH, alteration of temperature and glucose concentration rise originated in the body as a result of various disease conditions. Polymers, synthetic polypeptides and dendrimers have been used in the present work to study the feasibility of drug delivery. The computational methods have been used to formulate polymer properties, pharmacokinetics and toxicity studies. Diverse interactions approximating electrostatic, hydrophobic and hydrogen bond interactions acquire place during incorporation of drugs within the polymer and dendrimers. The covalent and electrostatic interactions between a drug and the surface of polymer and dendrimer have been analyzed. The docking interaction studies have been performed and the best polymer and dendrimer complex have been selected based on the docking score, binding energy and interaction energy with the drugs. G5 generation of poly amidoamine dendrimers and poly N-Ndiethyl acrylamide (PDEAAM) have been identified as most suitable stimuli-responsive effective drug carriers for anti diabetic drugs and diuretics. Favorable results have been obtained while using poly acrylic acid (PAA) for corticosteroids and polylysine for diabetic drugs. ConA protein along with poly aspartic acid also showed good results.

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