Qualification: 
Ph.D

Dr. Sahadev Shankarappa joined Amrita Center for Nanosciences and Molecular Medicine in May 2013. He is a physician - scientist with post-doctoral training in the laboratories of Professor Robert Langer at MIT, and Professor Daniel Kohane at Boston Children's Hospital, Harvard Medical School, Boston. Dr. Sahadev has PhD in Neurosciences from Loyola University, Chicago, Master of Public Health (M.P.H.) from the University of North Carolina at Chapel Hill, and M.B.B.S. from Bangalore University. He is also the recipient of the prestigious Ramalingaswami reentry fellowship, administered by the Department of Biotechnology; Government of India. Dr Sahadev is an author with multiple publications in reputed international journals. One of his works has been published in PNAS.

Dr. Sahadev's research interest is in the area of controlled drug delivery systems, specifically to the nervous system, to manage chronic pain. At ACNSMM, Dr. Sahadev continues to pursue his interest in developing novel nanotechnology-based delivery systems to treat neuropathic pain. His lab is working to develop novel nanoparticulate systems that can incorporate drugs, mRNA, and other therapeutic compounds of interest, directly into pain neurons. The goal of his research is to produce therapeutic options that are safe, affordable, and can be translated to the bedside, to treat various neuronal deficits.

Publications

Publication Type: Journal Article

Year of Publication Publication Type Title

2016

Journal Article

S. Padmakumar, Jipnomon Joseph, Neppalli, M. H., Mathew, S. E., Shantikumar V Nair, Dr. Sahadev Shankarappa, and Menon, D., “Electrospun Polymeric Core-sheath Yarns as Drug Eluting Surgical Sutures”, ACS Applied Materials and Interfaces, vol. 8, pp. 6925-6934, 2016.[Abstract]


Drug-coated sutures are widely used as delivery depots for antibiotics and anti-inflammatory drugs at surgical wound sites. Although drug-laden coating provides good localized drug concentration, variable loading efficiency and release kinetics limits its use. Alternatively, drug incorporation within suture matrices is hampered by the harsh fabrication conditions required for suture-strength enhancement. To circumvent these limitations, we fabricated mechanically robust electrospun core-sheath yarns as sutures, with a central poly-l-lactic acid core, and a drug-eluting poly-lactic-co-glycolic acid sheath. The electrospun sheath was incorporated with aceclofenac or insulin to demonstrate versatility of the suture in loading both chemical and biological class of drugs. Aceclofenac and insulin incorporated sutures exhibited 15% and 4% loading, and release for 10 and 7 days, respectively. Aceclofenac sutures demonstrated reduced epidermal hyperplasia and cellularity in skin-inflammation animal model, while insulin loaded sutures showed enhanced cellular migration in wound healing assay. In conclusion, we demonstrate an innovative strategy of producing mechanically strong, prolonged drug-release sutures loaded with different classes of drugs. © 2016 American Chemical Society. More »»

2015

Journal Article

A. Mohandas, Nimal, T. R., Das, V., Dr. Sahadev Shankarappa, Dr. Raja Biswas, and Dr. Jayakumar Rangasamy, “Drug Loaded Bi-layered Sponge for Wound Management in Hyperfibrinolytic Conditions”, Journal of Materials Chemistry B, vol. 3, pp. 5795-5805, 2015.[Abstract]


Excessive bleeding due to premature clot lysis and secondary bacterial wound infection are two significant problems that contribute to increased morbidity in patients with hyperfibrinolytic conditions. In this study, we have developed a bi-layered sponge that promotes fibrin clot stability and prevents secondary bacterial wound infections. Using the technique of freeze-drying, a bi-layer matrix consisting of hyaluronic acid (HA) containing aminocaproic acid (amicar) and chitosan containing tetracycline loaded O-carboxymethyl chitosan nanoparticles (Tet-O-CMC NPs) were produced. We hypothesized that the top chitosan layer with Tet-O-CMC NPs will prevent wound infection and concomitantly act as a matrix for cellular migration and subsequent wound healing, while the amicar-containing layer would promote clot stability. Tet-O-CMC NPs and bi-layer sponges were characterized using Dynamic Light Scattering (DLS), Scanning Electron Microscopy (SEM) and Fourier Transform Infra Red (FT-IR) spectroscopy. Physiochemical characterization such as porosity, swelling and mechanical testing was performed. The drug release study shows that the bi-layered sponge demonstrates a robust burst release of amicar and a sustained release of tetracycline. The ex vivo muscle permeation study indicated that Tet-O-CMC NPs have enhanced tissue permeation compared to free Tet. In vitro antibacterial activity of the bi-layer sponge towards laboratory and clinical strains of Staphylococcus aureus and Escherichia coli was proved. The ex vivo bacterial sensitivity study using porcine muscles confirmed the antibacterial activity, while the cell viability study using human dermal fibroblast (HDF) cells revealed its biocompatible nature. The in vitro antifibrinolytic study shows that the bi-layered sponge with amicar showed significant protection against streptokinase induced clot lysis. These studies suggest that the prepared amicar and tetracycline loaded chitosan-HA bi-layered sponge can be used effectively to promote better wound healing by simultaneously preventing bacterial infection, and enhancing clot stability. This journal is © The Royal Society of Chemistry 2015.

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