Qualification: 
M. Pharm.
Email: 
saranyats19347@aims.amrita.edu

Ms. Saranya T.S. joined Amrita School of Pharmacy in June 2013. She completed her Bachelor's Degree in Pharmacy from JKK Nataraja college of Pharmacy, The Tamil Nadu Dr. M.G.R. Medical University and her Master's Degree in Pharmaceutical Chemistry from Amrita School of Pharmacy. After obtaining her post graduation, she started her career in teaching in the year 2013. She joined Amrita School of Pharmacy from June 2013 onwards. She has 5 years of teaching and research experience and has several publications in national and international journals. Ms. Saranya's current research focuses on Insilico drug design, Medicinal chemistry drug design and Retrosynthesis. She is presently supervising M. Pharm. and B. Pharm. students in their research project on Computational Drug design and Medicinal Chemistry.

Publications

Publication Type: Journal Article

Year of Publication Publication Type Title

2017

Journal Article

S. .T.S, ,, Chittethu, A. B., Jose, A., Balasubramanian, R., and Asha Asokan Manakadan, “Utility of Isatin Semicarbazones in Mammary Carcinoma Cells - A Proof of Concept Study”, Journal of Young Pharmacists, 2017.[Abstract]


The present study was aimed to determine cytotoxic and apoptotic activity of isatin semicarbazones against mammary carcinoma. Methodology: The preparation of these target compounds involved substitution at the third position of the isatin nucleus along with chemical modification at the nitrogen atom of the scaffold to obtain more potent derivatives. The synthesized analogs were characterized by recording their melting point and Rf values along with IR, NMR and mass spectra. An antioxidant study and Cytotoxic Studies were performed by DPPH, Nitric oxide scavenging method and MTT assay methods. Results: The results revealed that all the test compounds (5a-5d) exhibited good anti oxidant activity. The synthesized compounds were then screened for in-vitro cytotoxicity by MTT cell proliferation assay method against breast cancer cell lines including MCF-7 and BT-549 with derivative 5a exhibiting maximum activity in both the cell lines. DNA fragmentation studies further indicated that 5a was involved in apoptotic process. Conclusion: Interestingly, it was observed that while all the synthesized compounds seemed active, 5a exhibited a superior ability to induce apoptosis. More »»

2017

Journal Article

A. Rajendran, Martin, S., Eso, R., Asha Asokan Manakadan, and .T.S, S., “Computational simulation studies of various flavonoid subclasses on treating non-small cell lung carcinoma associated with cigarette smoking”, Journal of Pharmaceutical Sciences and Research, vol. 9, pp. 1117-1121, 2017.[Abstract]


Lung cancer is one of the most prevailing cancers that cause death worldwide. Non-small cell lung cancer is getting tremendously increased day-by-day because of cigarette smoking. On exposure to the tobacco carcinogen- nicotine derived nitrosamine ketone (NNK) leads to the over expression of hepatocyte growth factor (HGF) and c-Met protein,that triggers processes such as metastasis, angiogenesis, anti-apoptosis, enhanced cell growth and motility. Methotrexate treats NSCLC; with major side effects leucopenia and inflammation. So it was essential to propose a novel drug having more potential, least side effects and better therapeutic efficacy. The uptake of flavonoid foods can reduce the risk and further progression of lung cancer. Apart from antitumor activity, flavonoids also possess blood cell production and anti-inflammatory actions. Hence c-Met protein resides attractive binding sites for the invention of new drugs for NSCLC by the incorporation of computational simulation tools and softwares. The docking analysis was performed using Arguslab for the identification of best ligands by predicting the ligand conformation in the protein active sites and assessment of binding affinities. Among 28 ligands were docked with the protein (pdb id: 5EOB) all had shown higher docking scores than the standard drug methotrexate. Thus, we can conclude that flavonoids can become a promising lead in designing a new and improved drug target that are beneficial in NSCLC therapy. Further analysis can be conducted for the synthesis of these drug targets to determine its actions in both in vivo and in vitro studies. © 2017, Pharmainfo Publications. All rights reserved.

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2017

Journal Article

T. James, Rajendran, A., Martin, S., Easo, R., Krishnan, N. C., Asha Asokan Manakadan, and .T.S, S., “Computational Approach on The Drug Affinity Studies of Substituted (4-Aminophenyl)Benzothiazole Derivatives Against Colon Cancer”, Research Journal of Pharmaceutical, Biological and Chemical Sciences (RJPBCS), vol. 8(2), pp. 402-407, 2017.[Abstract]


Colon cancer is the growth arising from the inner wall of colon, which is a part of large intestine. Wnt-signalling pathway has been recognized as a crucial factor in carcinogenesis. Mutations in this pathway lead to the accumulation of beta –catenin which initiates oncogenesis. Thus beta catenin is selected as the target to resist against the uncontrolled growth. In this work, a total of 10 substituted (4-aminophenyl) benzothiazole derivatives were selected for docking studies. These molecules were selected based on their potent antitumor, antifungal, antimicrobial, anthelmintic antidiabetic, and anticonvulsant, anti-inflammatory and antimalarial properties. The primary and secondary characterizations of protein(PDB ID:3SLA) were achieved from pdb structures using the aid of protparam and sopma tools.Insilico docking analysis were carried out, using Argus lab version 4.0 based on their scoring functions and Lipinski rule of 5 .It revealed that these derivatives have shown better docking score than the standard drug RALTITREXED; hence, can be used to reduce one of its side effect inflammation , and showed strong anticancer activity by the suppression of beta catenin, thus making them possible inhibitors against colon carcinogenesis. The main objective of our research work is to conclude that substituted (4-aminophenyl) benzothiazole derivatives are better drugs than RALTITREXED as it shows higher binding energy with the modeled protein. So these substituted derivatives can be used as suitable drug of choice against colon cancer.

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2016

Journal Article

V. Vishnu, .T.S, S., and Asha Asokan Manakadan, “In silico strategy for designing of novel chromene and indole derivatives to combat diabetes mellitus”, Research Journal of Pharmaceutical, Biological and Chemical Sciences, vol. 7, pp. 1098-1105, 2016.[Abstract]


Diabetes Mellitus is an assorted group of metabolic disorders due to the inability of pancreas to secrete insulin or the insensitivity of the cells to insulin. This work has been chosen considering the vital role of aldose reductase inhibitors in Diabetes Mellitus through the polyol pathway. Consequently an effective cont rol is the key to tackle this abnormal condition. In silico analysis for the development of aldose reductase inhibitors were carried out using both phytochemicals and generation of new lead molecules as ligands. The phytochemicals selected for the study were Allicin, Galegine, Rhamnoside, Quercetin, Trigonelline and Ferulic Acid. The lead molecule nucleus finalized were that of chromenes and indole derivatives. The present study included the analysis of parameters relating to proteins such as primary and s econdary structure analysis, subcellular location, analysis of cavities, ADME as well as docking results. Positive results were shown by Allicin, Galegine, Rhamnoside, Quercetin, Trigonelline and Ferulic Acid when compared with the standard drugs like Epalrest and Sorbinil. The lead molecules of chromeme derivative AR2,methyl2,2-dimethyl3-(4oxo-3-phenoxymethyl)-4H-chromrn-8-yl)propanoate and indole derivative AR7, methylidene(([1-(phenoxymethyl)-2,3-dihydro-1H-indol-5yl]oxy))amine screened displayed significant results over the phytochemicals and the standard drugs used. Further in vitro pharmacological activities might prove to be useful to substantiate the results.

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2016

Journal Article

A. S. Sekhar, .T.S, S., Baskar, V., and Asha Asokan Manakadan, “In Silico Approach of Apoptosis Inducing Ability of Different Indole Derivatives by Interaction with CASPASE9”, Int. J. Pharm. Sci. Rev. , vol. 40, no. 2, pp. 92-102 , 2016.[Abstract]


Indole compounds are well known for their wide variety of pharmacological activities. It is the combination of benzene with pyrrole
ring. Compounds having indole group are biologically important. They are used as antimicrobial, antiviral, antituberular, antiinflammatory,
anticancer, ant-diabetic and anticonvulsant agents. Because of the wide variety of biological application, several
substituted indole derivatives are studied for their molecular structure, molecular docking and bioavailability. The purpose of the
study is to carry out the docking studies of indole derivatives containing electrophilic substitution and nucleophilic substitution with
the anticancer target casp9. Fifty compounds were designed and by using Argus lab version 4.0.1. Their docking score was calculated
and compared with the standard drug casodex. The drug likeness of compounds was performed using Lipinski rule of five. Result
showed that 1,1’-(1H-indole-5,6-diyl)diethanone and 5-(trichloromethyl)-1H-indole and the phytoconstituent curcumin showed
better docking score than that of the standard drug casodex. It is concluded that certain indole derivatives show greater affinity with
casp9 protein. These compounds may be helpful in studying anticancer targets for casp9 protein.

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2016

Journal Article

N. Prasad Nair, Joy, J., Kumar, S. S., .Sathianarayanan, S., Asha Asokan Manakadan, and .T.S, S., “In-silico Docking Studies of Coumarin Derivatives as Caspase 8 and PDE4 Antagonist.”, Research Journal of Pharmacy and Technology , 2016.[Abstract]


Coumarin, a well-known organic compound for its wide range of activities, incorporating a benzene and pyrone ring together, belongs to benzopyrone family. Piroxicam is the drug that is used in the treatment of inflammation as well as cancer but it possess certain side effects like constipation, blurred vision etc. Coumarin shows anti-inflammatory and anticancer activity based on different substitution on it. The purpose of this study is to screen the best target among Caspase-8 and PDE4. Arguslab 4.0.1 docking analysis was performed to find out the best ligand which shows highest score for anti-inflammatory and anti-cancer activity and compared with the standard drug piroxicam. The phytoconstituents like isofraxidin and scopoletin having coumarin pharmacophore were also used for docking analysis. Among the twenty eight analogues of coumarin, twenty four showed good score for caspase 8 and all compounds possess good score for PDE4 compared to piroxicam. The best target among caspase 8 and PDE4 for anti- inflammatory and anti-cancer activity was found to be PDE4 and ethyl substitution at the 7th position of coumarin derivatives showed good affinity against PDE4.

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2015

Journal Article

Asha Asokan Manakadan, .T.S, S., S, S. K., and J, C., “A Computational In silico Approach for Identification of Indian Herbs for the Treatment of Alzheimer's Disease”, Research Journal of Pharmaceutical, Biological and Chemical Sciences, vol. 6, no. 1, pp. 414-422, 2015.[Abstract]


Alzheimer's disease (AD) is a neuronal disorder associated with dementia and EphB2 gene play a crucial role in the NMDA signaling pathway helping to restore cognitive functions. Therefore, attempt has been made to study the pharmacogenomics of the gene to identify the individual variations by the functional effect of Single Nucleotide Polymorphisms (SNPs). Out of the 4997 SNPs, 9 SNPs were found to be damaging in nature with deleterious amino acid substitutions. Computer Aided Drug Designing (CADD) was instrumental to find out the effective constituent among the plant sources, Withania somnifera, Centella asiatica, Catharanthus roseus, Curcuma longa and Bacopa monnieri with Anti-Alzheimer Activity. The corresponding 21 plant constituents analyzed were Withanolide E, Withanolide A, Withanoside IV, Withaferine A, Anaferine, Beta-Sitosterol, Quercetin, Asiatic acid, Asiaticoside, Campesterol, Madecassoside, Madecassic acid, Vincristine, Vincamine, Vinblastine, Catharanthine, Kaempferol, Curcumin, Ascorbic acid, Linalool and Bacoside A. Molecular property, bioactivity parameter as well as Protein-Ligand Dynamic interaction were analyzed to determine the drug likeness of the selected constituents. Bench marking were done using an antioxidant Butylated hydroxytoluene (BHT). The in silico analysis performed indicate the selection of natural compounds like Withanoside IV, Asiaticoside, Madecassoside, Vincristine, Vinblastine and Bacoside A to show significant binding interaction with 1B4F protein.

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2015

Journal Article

S. G. Gayathri, Vishnu, V., Shibu, S., .T.S, S., and Asha Asokan Manakadan, “Computational identification and analysis of phytoconstituents inhibiting vital proteins in Mycobacterium tuberculosis”, Journal of Chemical and Pharmaceutical Research, vol. 7, no. 11, pp. 170-176, 2015.[Abstract]


Tuberculosis caused by Mycobacterium tuberculosis is disproportionately one of the major burden in health care system. In the present work we have extensively studied the target proteins Pantothenate synthetase, Thymidylate kinase and Filamentous temperature sensitive protein Z (FtsZ) for its significance in the treatment for tuberculosis. Computer aided drug design (CADD) aided to predict the most effective phytoconstituents for the tre3atment for tuberculosis among the 28 plant sources used for the study. The phytoconstituents taken up for the investigation included Agnuside, Alloin, Alliin, Alpha-amyrin, Alizarin, Aloe-emodin, Berberine, Coumaric acid, Kaempherol, Kumatakenin, Luteolin, Mimosine, Mangiferin, Myricetin, Niacin, Oleanane, Orientin, Protocatechuic acid, Piperine, Quinoline, Rottlerin, Sesamin, Scopoletin, Serotonin, Taraxerol, Thiamine, Ursolic acid and Xanthone. The computational approach included the prediction of Molecular properties, bioactive scores, the analysis of primary and secondary structures of proteins and the binding interaction calculation using docking principles. The results of in silico study pointed out that the phytoconstituents such as Alizarin, Kaempherol, Myricetin, Alliin, Coumaric acid, Protocatechuic acid, Aloin, Agnuside, Aloe-emodin, Mangiferin and Rottlerin elicits good interaction by inhibiting the selected proteins as compared with the standard drug Isoniazid. Further investigation is essential for the development of potent inhibitors for the control of the disease.

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2015

Journal Article

Dr. S. Sathianarayanan, S, S. M., Kumar, R. S., V, S., Menon, S. V., Mohan, S., .T.S, S., Asha Asokan Manakadan, and Suja, S., “A Computational approach for identification of Phytochemicals for targeting and optimizing the inhibitors of Heat shock proteins”, Research Journal of Pharmacy and Technology., vol. 8, no. 9, pp. 1199- 1204, 2015.[Abstract]


Pancreatic cancer is a class of disease characterized by uncontrolled cell growth that begins in the pancreas. Heat shock proteins (Hsps) are chaperone proteins that on inhibition can prove to be effective in pancreatic cancer treatment. In cancer cells, Hsp90 significantly displays a proliferative potential of the malignant cells, so the inhibition of the protein can prove to be valuable to combat pancreatic cancer. Computer Aided Drug Designing (CADD) was instrumental to find out the effective constituent among the 35 plant sources selected. The Phytochemicals used for the present study were Curcumin, Allicin, Pinene, Tetrahydrocannabinol, Astragalin, Arginine, Scopoletin, Baicalin, Stylopine, Carvacrol, Quercetin, Thymol, Guaiaretic acid, Coumarin, Chlorogenic acid, Taraxacin, Ascorbic acid, Protocatechuic acid, Withaferin A, Triptolide, Ursolic acid, Cucurbitane, Thymoquinone, D-Carvone, Eugenol, Ellagic acid, Lapachol, Genistein, Emodin, Chelidonine, Caffeine, Catechin, Geraniol, Myrcene and Niacin. The preliminary studies such as the Primary and Secondary structure analysis of the protein were carried out. Molecular property based on Lipinski rule of 5, bioactivity parameters as well as Protein-Ligand Dynamic interaction were analyzed to determine the drug likeness of the ligands with the protein 4L94. The results of the in silico docking analysis indicate the selection of natural compounds such as Cucurbitane, Myrcene and Pinene as they elicited significant binding interaction in inhibiting Heat shock proteins for targeting pancreatic cancer which was comparable with that of the standard drug Gemcitabine. Further in vivo studies may be carried out to prove the same.

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2015

Journal Article

R. Thomas, Hari, R., Joy, J., Krishnan, S., A.N, S., Nair, S. S., Asha Asokan Manakadan, ,, and .T.S, S., “In silico Docking Approach of Coumarin Derivatives as an Aromatase Antagonist”, Research Journal of Pharmacy and Technology , vol. 8, no. 12, pp. 1673-1678 , 2015.[Abstract]


<p>Coumarins are pharmacologically active moiety well known for their wide variety of activities. Coumarin is the combination of benzene and pyrone ring and it belongs to benzo pyrone family. It is available from plants, microorganisms etc. Depending on various substitutions on different positions of coumarin, we get different pharmacological properties like anticancer, antioxidant, antifungal, anti-inflammatory, antiviral, anticoagulant etc. The purpose of this study is to carry out the docking studies of coumarin derivatives containing electrophilic and nucleophilic substitutions with the anticancer target aromatase by using Arguslab version 4.0.1. Fifty four lead molecules were designed and their docking scores were compared with the standard drug Gefitinib. The validation of ligands were performed using Lipinski rule of five. It was observed that nineteen ligands showed better docking score than the standard drug Gefitinib. Phytoconstituents like Isofraxidin, Scopoletin and umbelliferone from Compositae family, which were the source of coumarin nucleus were also taken for the docking analysis. It was observed that most of the lead molecules showed higher docking score than the phytoconstituents.</p>

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2014

Journal Article

Asha Asokan Manakadan, .T.S, S., and , “In silico analysis of functional snps of Alox12 gene and identification of pharmacologically significant flavonoids as lipoxygenase inhibitors”, International Research Journal of Pharmacy , vol. 5, no. 6, pp. 502-507, 2014.[Abstract]


Cancer is a disease affecting any part of the body and in comparison with normal cells there is an elevated level of lipoxygenase enzyme in different cancer cells.

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2013

Journal Article

A. Jose, Chittethu, A. Balakrishn, Sankaran, S., .T.S, S., and Ekambaram, K. Prem, “Synthesis and characterization of quinazolinone derivatives against mammary carcinoma”, Journal of Pharmacy Research, vol. 6, no. 9, pp. 933 - 938, 2013.[Abstract]


Aim
The present study was aimed to synthesis series of quinazolinone derivatives against mammary carcinoma.

Methods
The compounds were synthesized by reaction of nucleophilic attack, Wohler's classical synthesis followed by condensation reaction. The synthesized quinazolinone derivatives were characterized by physico chemical analysis like melting point, TLC, IR, UV and NMR. Compounds have been evaluated for their in-vitro antioxidant methods like DPPH method, H2O2 method, nitrous oxide scavenging method, super oxide scavenging method, ABTS radical scavenging, lipid peroxidation and also in-vitro cytotoxicity study against MCF-7, BT-549, ZR-75 (human breast cancer) cell lines by MTT assay method.

Results
From physico chemical data the structure of synthesized compounds was confirmed. Antioxidant activity was found from the IC50 value. Qc showed lowest IC50 value in DPPH radical scavenging assay and nitric oxide scavenging assay and was observed to be 27.20 μg/ml and 26.3 μg/ml respectively. In super oxide scavenging activity and lipid peroxidation and H2O2 assays showed a good activity it was observed 26.3 μg/ml, 18.10 μg/ml and 21.4 μg/ml respectively. Qb showed lowest IC50 value in ABTS radical scavenging assay and was observed to be 26.9 μg/ml. Qe showed lowest IC50 value in lipid peroxidation method and was observed to be 149 μg/ml. QN-D showed a moderate antioxidant. In cytotoxic activity, Qc showed lowest IC50 value, i.e. 21.77, 23.03 and 21.14 μg/ml and Qb showed moderate values like 23.25 μg/ml, 27.39 μg/ml and 27.86 μg/ml in MCF-7, BT-549 and ZT-75 respectively.

Conclusion
All the synthesized compounds showed moderate to good antioxidant and potent cytotoxic activity.

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2012

Journal Article

S. .T.S, Parvathy, N. G., Padma, R., Renjith, V., and Rahate, K. P., “Phytochemical screening and anthelmintic activity of methanolic extract of Imperata cylindrica”, International journal of Pharmacy and pharmaceutical science, vol. 4, no. 1, 2012.[Abstract]


The study aimed to investigate potential in vitro anthelmintic activity of methanolic extract of roots of Imperata cylindrica (Poaceae) against Indian
earthworms Pheretima posthuma. Various concentrations (10-80 mg/50ml) of the extract were tested, which involved determination of time of paralysis
and time of death of the worms. The methanolic extract exhibited a maximum anthelmintic activity (p<0.01) comparable to standard drug albendazole
(1000 mg/50ml). The extract showed anthelmintic activity in dose dependant manner giving shortest time of paralysis (P) and death (D) with 80mg/ml
concentration and caused paralysis in 3.3 min and death in 6.0 min. The Preliminary phytochemical analysis indicated the presence of various
phytoconstituents in the extract of which tannins and saponins might have contributed for the potent anthelmintic activity.

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2010

Journal Article

D. Raj, .C, S., .C, D., .A.K, A., Mani, L. Mary, Mathew, L., Mambra., A. Bari M., .V, S., .T.S, S., .K.C, S., and .S, U., “Evaluation of the suspending properties of Leucaena Latisiliqua seed gum”, Der pharmacia lettre, vol. 2, no. 5, pp. 67-74, 2010.[Abstract]


The purpose of the study is to select a new cheap, effective alternative natural suspending agent for pharmaceutical suspensions. Comparative study between the Leucaena Latisiliqua seed gum and known gums like Tragacanth, Acacia were done. Leucaena Latisiliqua seed was boiled and the seed gum was extracted with acetone, dried and powdered. Then its phytochemical investigation, swelling index was determined. Different type of zinc oxide suspensions using Leucaena Latisiliqua seed gum, Tragacanth, Acacia were prepared and effect of type and concentration of suspending agent on sedimentation volume, viscosity and particle size were studied. The phytochemical test showed the presence of less common sugar. The zinc oxide suspension prepared in batches containing Leucaena gum, compound tragacanth, acacia and the result showed that sedimentation volume, viscosity, and the particle size were found directly proportional to the concentration of suspending agent and inversely proportional to the flow rate. The suspending ability of the suspending agents were in the order of Leucaena Latisiliqua gum > Compound Tragacanth >Acacia. From the results, it was concluded that the gum of Leucaena Latisiliqua can be employed as stabilizer and thickener of choice when high viscosity is desired.

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