Brain proteomics of anopheles gambiae
Publication Type:Journal Article
Source:OMICS A Journal of Integrative Biology, Mary Ann Liebert Inc., Volume 18, Number 7, p.421-437 (2014)
Keywords:adult, alternative RNA splicing, amino acid sequence, animal experiment, animal tissue, Anopheles gambiae, article, brain mapping, brain protein, brain tissue, chromatin assembly and disassembly, female, G protein coupled receptor, liquid chromatography, malaria, male, nonhuman, priority journal, protein database, protein synthesis, proteome, proteomics, reverse transcription polymerase chain reaction, sequence analysis, synapse vesicle, Synaptic Transmission, tandem mass spectrometry, transport vesicle
Anopheles gambiae has a well-adapted system for host localization, feeding, and mating behavior, which are all governed by neuronal processes in the brain. However, there are no published reports characterizing the brain proteome to elucidate neuronal signaling mechanisms in the vector. To this end, a large-scale mapping of the brain proteome of An. gambiae was carried out using high resolution tandem mass spectrometry, revealing a repertoire of >1800 proteins, of which 15% could not be assigned any function. A large proportion of the identified proteins were predicted to be involved in diverse biological processes including metabolism, transport, protein synthesis, and olfaction. This study also led to the identification of 10 GPCR classes of proteins, which could govern sensory pathways in mosquitoes. Proteins involved in metabolic and neural processes, chromatin modeling, and synaptic vesicle transport associated with neuronal transmission were predominantly expressed in the brain. Proteogenomic analysis expanded our findings with the identification of 15 novel genes and 71 cases of gene refinements, a subset of which were validated by RT-PCR and sequencing. Overall, our study offers valuable insights into the brain physiology of the vector that could possibly open avenues for intervention strategies for malaria in the future. © Copyright 2014, Mary Ann Liebert, Inc. 2014.
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Cite this Research Publication
S. Bab Dwivedi, Muthusamy, Bac, Kumar, Pa, Kim, M. - Sd, Nirujogi, R. Sac, Getnet, Dd, Ahiakonu, Pe, De, Gaf, Dr. Bipin G. Nair, Gowda, Ha, Prasad, T. S. Kab c f, Kumar, Ng, Pandey, Aad h, and Okulate, Me, “Brain proteomics of anopheles gambiae”, OMICS A Journal of Integrative Biology, vol. 18, pp. 421-437, 2014.