Publisher Summary This chapter presents a number of experimental approaches that have proved to be very useful in elucidating the mechanism(s) involved in epidermal growth factor (EGF) -mediated stimulation of cardiac adenylylcyclase. However, there are several additional questions pertaining to the detailed understanding of the mechanism(s) involved in interactions between the EGF receptor and Gs interaction. Thus, the in vitro studies demonstrate the juxtamembrane region of the EGF receptor that is important for stimulation of Gs by performing mutation(s) of key residue(s) in the juxtamembrane region of the EGF receptor, to determine whether the ability of EGF to stimulate adenylylcyclase can be obliterated. Similarly, it is important to determine the region(s) of Gsα that interact with the EGF receptor. It is also determine that what elements confer specificity to the ability of EGF to stimulate adenylylcyclase. The latter goal poses a particularly important question, because EGF does not elevate cAMP content in all cells that express the EGF receptor, Gs, and adenylylcyclase.
Tarun B. Patel, Hui Sun, Helen Poppleton, Dr. Bipin G. Nair, Hani M. Rashed, and Yi-Ming Yu, “ Epidermal Growth Factor-mediated Regulation of G Proteins and Adenylylcyclase in Cardiac Muscle”, in G Proteins, vol. 29, P. C. Roche, Ed. Academic Press, 1996, pp. 319 - 343.