5-Fluorouracil loaded fibrinogen nanoparticles for cancer drug delivery applications
Publication Type:Journal Article
Source:International Journal of Biological Macromolecules, Volume 48, Number 1, p.98-105 (2011)
Keywords:animal cell, Animals, Antineoplastic Agents, article, atomic force microscopy, calcium chloride, cancer cell culture, cattle, Cell death, Cell Line, cell strain L 929, cell strain MCF 7, coacervation, controlled study, cross linking, differential scanning calorimetry, drug cytotoxicity, drug delivery system, Drug Delivery Systems, drug release, drug uptake, fibrinogen, fibrinogen nanoparticle, Fluorescence, fluorouracil, Fourier Transform Infrared, human, human cell, Humans, in vitro study, infrared spectroscopy, Light, light scattering, microscopy, mouse, nanoparticle, Nanoparticles, nonhuman, particle size, prostate cancer, Radiation, Rhodamine 123, Rhodamines, scanning electron microscopy, Scattering, Spectroscopy, static electricity, temperature, Thermogravimetry, Tumor, ultraviolet spectrophotometry, unclassified drug, X ray diffraction, X-Ray Diffraction, zeta potential
In this study, 5-flurouracil loaded fibrinogen nanoparticles (5-FU-FNPs) were prepared by two step coacervation method using calcium chloride as cross-linker. The prepared nanoparticles were characterized using DLS, SEM, AFM, FT-IR, TG/DTA and XRD studies. Particle size of 5-FU-FNPs was found to be 150-200 nm. The loading efficiency (LE) and in vitro drug release was studied using UV spectrophotometer. The LE of FNPs was found to be ∼90%. The cytotoxicity studies showed 5-FU-FNPs were toxic to MCF7, PC3 and KB cells while they are comparatively non toxic to L929 cells. Cellular uptake of Rhodamine 123 conjugated 5-FU-FNPs was also studied. Cell uptake studies demonstrated that the nanoparticles are inside the cells. These results indicated that FNPs could be useful for cancer drug delivery. © 2010 Elsevier B.V.
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