Amidase, a cell wall hydrolase, elicits protective immunity against Staphylococcus aureus and S. epidermidis
Publication Type:Journal Article
Source:International Journal of Biological Macromolecules, Elsevier, Volume 77, p.314-321 (2015)
Keywords:amidase, animal cell, animal experiment, animal model, animal tissue, antibody production, article, bacterial cell wall, bacterial strain, biofilm, cell adhesion, cell division, cell separation, Cell Surface, cellular immunity, controlled study, drug design, drug identification, extracellular matrix, Freund adjuvant, immunoglobulin antibody, immunoglobulin G2a antibody, immunoglobulin G2b antibody, infection prevention, Mus, n acetylmuramylalanyl amidase vaccine, nonhuman, Staphylococcus aureus, Staphylococcus epidermidis, Staphylococcus infection, Staphylococcus vaccine, Th1 cell, Th2 cell, unclassified drug
The morbidity and the mortality associated with Staphylococcus aureus and S. epidermidis infections have greatly increased due to the rapid emergence of highly virulent and antibiotic resistant strains. Development of a vaccine-based therapy is greatly desired. However, no staphylococcal vaccine is available till date. In this study, we have identified Major amidase (Atl-AM) as a prime candidate for future vaccine design against these pathogens. Atl-AM is a multi-functional non-covalently cell wall associated protein which is involved in staphylococcal cell separation after cell division, host extracellular matrix adhesion and biofilm formation. Atl-AM is present on the surface of diverse S. aureus and S. epidermidis strains. When used in combination with Freund's adjuvant, Atl-AM generated a mixed Th1 and Th2 mediated immune response which is skewed more toward Th1; and showed increased production of opsonophagocytic IgG2a and IgG2b antibodies. Significant protective immune response was observed when vaccinated mice were challenged with S. aureus or S. epidermidis. Vaccination prevented the systemic dissemination of both organisms. Our results demonstrate the remarkable efficacy of Atl-AM as a vaccine candidate against both of these pathogens. © 2015 Elsevier B.V.
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