Publication Type:

Journal Article

Source:

Pharmaceutical Research, Springer New York LLC, Volume 31, Number 5, p.1338-1351 (2014)

URL:

http://www.scopus.com/inward/record.url?eid=2-s2.0-84902267132&partnerID=40&md5=b029271f3b76dc50f7421b813f1d013c

Keywords:

Alkalinity, antibacterial activity, antifungal activity, article, Candida albicans, cell viability, ciprofloxacin, controlled study, cross linking, Diffusion, drug delivery system, drug release, emulsion, Escherichia coli, Fibrin, fibrin nanoparticle, fibrinogen, fluconazole, human, human cell, in vitro study, nanoencapsulation, nanoparticle, nonhuman, physical chemistry, priority journal, Staphylococcus aureus, synthesis, thrombin, unclassified drug, vascularization, water oil cream, Wound healing, wound infection

Abstract:

Purpose: In vitro evaluation of antibacterial and antifungal drugs encapsulated fibrin nanoparticles to prove their potential prospect of using these nanocomponent for effective treatment of microbial infested wounds. Methods: Surfactant-free oil-in-water emulsification-diffusion method was adopted to encapsulate 1 mg/ml each of antimicrobial drugs (Ciprofloxacin and Fluconazole) in 4 ml of aqueous fibrinogen suspension and subsequent thrombin mediated cross linking to synthesize drug loaded fibrin nanoparticles. Results: Ciprofloxacin loaded fibrin nanoparticles (CFNPs) showed size range of 253∈±∈6 nm whereas that of Fluconazole loaded fibrin nanoparticles (FFNPs) was 260∈±∈10 nm. Physico chemical characterizations revealed the firm integration of antimicrobial drugs within fibrin nanoparticles. Drug release studies performed at physiological pH 7.4 showed a release of 16% ciprofloxacin and 8% of fluconazole while as the release of ciprofloxacin at alkaline pH 8.5, was 48% and that of fluconazole was 37%. The antimicrobial activity evaluations of both drug loaded systems independently showed good antibacterial activity against Escherichia coli (E.coli), Staphylococcus aureus (S. aureus) and antifungal activity against Candida albicans (C. albicans). The in vitro toxicity of the prepared drug loaded nanoparticles were further analyzed using Human dermal fibroblast cells (HDF) and showed adequate cell viability. Conclusion: The efficacies of both CFNPs and FFNPs for sustained delivery of encapsulated anti microbial drugs were evaluated in vitro suggesting its potential use for treating microbial infested wounds (diabetic foot ulcer). © 2013 Springer Science+Business Media New York.

Notes:

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Cite this Research Publication

B. M. Alphonsa, Kumar, P. T. Sudheesh, Praveen, G., R.a Biswas, Chennazhi, K. P., and Jayakumar, R., “Antimicrobial drugs encapsulated in fibrin nanoparticles for treating microbial infested wounds”, Pharmaceutical Research, vol. 31, pp. 1338-1351, 2014.