Publication Type:

Journal Article

Source:

International journal of Engineering research and technology, Volume 3, Number 7 (2014)

Keywords:

(pdms), Atomic, dimethyl, DNA, force, lithography, lithography.benchtop, microcontact, microscopy, molecule, nanoscale, patterning, poly, printing, siloxane, soft, using

Abstract:

<p>NA microarrays have rapidly evolved to become one of the essential tools to examine Expression or mutation of thousands of genes simultaneously. The microcontact printing (�¼CP) could fulfill this requirement as it is a printing technology that uses inexpensive Elastomeric stamps made usually of polydimethylsiloxane (PDMS) and which exhibits relief patterns at the micron and Nano scale. In this work we made a benchtop nanoscale pattering using soft lithographic techniques and made a PDMS stamp. This stamp is characterized using Atomic force microscope and Laser diffraction technique to confirm the nanoscale patterns. Genomic and plasmid DNA (Maxprep) are isolated from Ecoli cells and these biomolecules are immobilized on solid surfaces such as surface modified microscope glass slide and silicon wafers by micro contact printing. These printed surfaces are characterized and analyzed by Atomic Force Microscopy (AFM). The surface bound biomolecules find application in bio sensors, chromatography, diagnostic immune assays, cell culturing, expression studies and other analytical procedures.</p>

Cite this Research Publication

V. Kumar. A, M, M., Ashok, A., Sreekala, C. Ob, and Rasheed, R., “Benchtop Nanoscale Patterning using Soft Lithography for the Printing of DNA Molecules”, International journal of Engineering research and technology, vol. 3, 2014.