Publication Type:

Journal Article

Source:

ACS Sustainable Chemistry and Engineering, American Chemical Society, Volume 6, Number 6, p.7826-7840 (2018)

URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-85046248692&doi=10.1021%2facssuschemeng.8b00915&partnerID=40&md5=04f8b17694fc1d5776c948a40feb6bdf

Keywords:

Anti-bacterial activity, Bacterial Infections, Cardiovascular surgery, chitin, Endothelial cells, Escherichia coli, Escherichia coli (E. coli), Gelatin nanoparticles, Human umbilical vein endothelial cells, Methicillin-Resistant Staphylococcus aureus, Mixtures, Sprague-Dawley rats, Staphylococcus aureus, Syringes, Transplantation (surgical)

Abstract:

Mediastinitis occurs after cardiac surgery and is a major threat to patient's life due to postoperative bleeding and deep sternal wound infection. Major challenge in treating this condition is that it demands a material that should adhere to the applied site and act as both a hemostatic and an antibacterial agent. On the basis of this we have developed an in situ forming tissue adhesive chitin-fibrin (CH-FB) gel with tigecycline loaded gelatin nanoparticles (tGNPs) for controlling bleeding and preventing bacterial infection. Spherical shaped tGNPs (231 ± 20 nm) were prepared and characterized. In situ forming tGNPsCH-FB gel was formed using a dual syringe applicator in which one syringe was loaded with a mixer of fibrinogen solution, chitin gel, and tGNPs; the other syringe was loaded with a mixture of thrombin solution, chitin gel, and tGNPs. Both these mixtures were injected together. In situ gel formed within a minute and exhibited excellent tissue adhesive property. tGNPsCH-FB gel was found to be cyto-compatible against human umbilical vein endothelial cells (HUVECs). Sustained release of tigecycline from tGNPsCH-FB gel was found to occur over 21 days. In vitro antibacterial activity of tGNPsCH-FB gel was tested against Staphylococcus aureus, methicillin-resistant Staphylococcus aureus (MRSA), Escherichia coli (E. coli), and their clinical isolates. Furthermore, in vivo hemostatic potential of tGNPsCH-FB gel was evaluated in deep organ injuries created in Sprague-Dawley rats. The developed gel exhibited rapid blood clotting potential by achieving hemostasis within 154 and 84 s under femoral artery (pressured) and liver (oozing) bleeding conditions. Hence, these findings exhibit the potential application of the developed tGNPsCH-FB gel to adhere at surgical site for controlling bleeding and prevent bacterial infection after cardiac surgery. © 2018 American Chemical Society.

Notes:

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Cite this Research Publication

M. N. Sundaram, V. Kaliannagounder, K., Selvaprithiviraj, V., Suresh, M., Biswas, R., Vasudevan, A. K., Varma, P. K., and Dr. Jayakumar Rangasamy, “Bioadhesive, Hemostatic, and Antibacterial in Situ Chitin-Fibrin Nanocomposite Gel for Controlling Bleeding and Preventing Infections at Mediastinum”, ACS Sustainable Chemistry and Engineering, vol. 6, pp. 7826-7840, 2018.