Publication Type:

Journal Article

Source:

Carbohydrate Polymers, Volume 83, Number 2, p.776-786 (2011)

URL:

http://www.scopus.com/inward/record.url?eid=2-s2.0-78349312099&partnerID=40&md5=b821c3123f53fefccbe3a697c45cd2b5

Keywords:

5-fluorouracil, apoptosis, Apoptosis assays, Biological materials, Cancer cells, Cancer drug delivery, Cell culture, Cell death, Cell lines, Cell uptake, chitin, chitosan, Concentration ranges, Crosslinking reaction, Cytotoxicity assays, Drug delivery, Grafting (chemical), Green fluorescence, In-vitro, LCST, Lower critical solution temperature, Nanoparticles, Polymeric nanoparticles, Polymers, Thermo sensitive, Thermo-responsive, toxicity

Abstract:

We developed a nanoformulation of 5-FU (5-fluorouracil) with biodegradable thermo-responsive chitosan-g-poly(N-vinylcaprolactam) biopolymer composite for its delivery to cancer cells. The novel thermo-responsive graft co-polymeric nanoparticles (TRC-NPs) were prepared by ionic cross-linking method, which showed a lower critical solution temperature (LCST) at 38 °C. The 5-FU drug was incorporated into the carrier using cross-linking reaction. The in vitro drug release showed prominent release above LCST. Cytotoxicity assay showed TRC-NPs in the concentration range of 100-1000 μg/ml are non-toxic to an array of cell lines. The drug-loaded nanoparticles showed comparatively higher toxicity to cancer cells while they are less toxic to normal cells. The cell uptake of the 5-FU loaded thermo-responsive graft co-polymeric nanoparticles (5-FU-TRC-NPs) was confirmed from green fluorescence inside cells by rhodamine-123 conjugation. The apoptosis assay showed increased apoptosis of cancer cells when treated with 5-FU compared to the normal cells. These results indicated that novel 5-FU-TRC-NPs could be a promising candidate for cancer drug delivery. © 2010 Elsevier Ltd. All rights reserved.

Notes:

cited By (since 1996)27

Cite this Research Publication

N. Sa Rejinold, Chennazhi, K. Pa, Nair, S. Va, Tamura, Hb, and Jayakumar, Ra, “Biodegradable and thermo-sensitive chitosan-g-poly(N-vinylcaprolactam) nanoparticles as a 5-fluorouracil carrier”, Carbohydrate Polymers, vol. 83, pp. 776-786, 2011.