A bioreductant-resistant 'turn-on' chemodosimetric fluorescent probe Mito-1 has been developed for the detection of mitochondrial HNO in live cells. Mito-1 enables the detection of HNO as low as ∼18 nM. It has the capability to detect both exogenous and endogenous mitochondrial HNO formations in cellular milieus by providing fluorescence images. Its two-photon imaging ability fosters its use as a noninvasive imaging tool for the detection of mitochondrial nitroxyl.
K. Sunwoo, Bobba, K. Naidu, Lim, J. - Y., Park, T., Podder, A., Heo, J. Seok, Lee, S. Gwan, Bhuniya, S., and Kim, J. Seung, “A bioorthogonal 'turn-on' fluorescent probe for tracking mitochondrial nitroxyl formation.”, Chemical Communications (Camb), vol. 53, no. 10, pp. 1723-1726, 2017.