Publication Type:

Journal Article

Source:

Materials Science and Engineering: C, Volume 85, p.239 - 248 (2018)

URL:

http://www.sciencedirect.com/science/article/pii/S0928493117333738

Keywords:

Bone tissue engineering, Critical sized defects, Genetic engineering, Mesenchymal Stem Cells, Plasmid based transfection

Abstract:

The treatment of critical sized bone defect remains a significant challenge in orthopedics. The objective of the study is to evaluate the effect of the combination of bone morphogenetic protein 2 (BMP2) expressing genetically engineered mesenchymal stem cells (MSCs) [MSCs engineered using a multimam vector, pAceMam1, an emerging gene delivery vector] and an osteoconductive scaffold [silica coated nanohydroxyapatite-gelatin reinforced with fibers] in enhancing bone regeneration in critical sized segmental defects. The scaffold with transfected MSCs showed significantly higher viability, proliferation and osteogenic differentiation in vitro. Further, this group augmented union and new bone formation in critical sized rat femoral segmental defect at 12 weeks when compared to control groups (scaffold with MSCs and scaffold alone). These data demonstrated that the MSCs engineered for transient expression of BMP2 can improve the repair of segmental defects, which paves an avenue for using pAceMam1 as a vector for bone tissue regeneration.

Cite this Research Publication

S. Kuttappan, Anitha, A., Minsha Mallika Gopi, Menon, P. M., Sivanarayanan, T. B., Dr. Lakshmi Sumitra, and Dr. Manitha B. Nair, “BMP2 Expressing Genetically Engineered Mesenchymal Stem Cells on Composite Fibrous Scaffolds for Enhanced bone Regeneration in Segmental Defects”, Materials Science and Engineering: C, vol. 85, pp. 239 - 248, 2018.

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