Herein, we report rational design, synthesis, and application of a two-photon fluorescent probe (Tyro-1) for tracking intracellular tyrosinase activity. The chemoselective detection of tyrosinase is precluded from interference of other competitive omnipresent oxidizing entities in cellular milieu. The probe showed 12.5-fold fluorescence enhancement at λ = 450 nm in the presence of tyrosinase. The nontoxic probe Tyro-1 provides information about HO-mediated upregulation of tyrosinase through cellular imaging. Its two-photon imaging ability makes it a noninvasive tool for validating the expression of tyrosinase in the live cells.
K. Naidu Bobba, Won, M., Shim, I., Velusamy, N., Yang, Z., Qu, J., Kim, J. Seung, and Bhuniya, S., “A BODIPY-based two-photon fluorescent probe validates tyrosinase activity in live cells.”, Chemical Communications, vol. 53, no. 81, pp. 11213-11216, 2017.