Publication Type:

Journal Article

Source:

Nanomedicine: Nanotechnology, Biology, and Medicine, Elsevier Inc., Volume 12, Number 5, p.1347-1355 (2016)

URL:

https://www.scopus.com/inward/record.url?eid=2-s2.0-84963700630&partnerID=40&md5=ebe297823aeafc777b74e7ded683b8af

Keywords:

Assays, Biomedical applications, cDNA microarray analysis, Cell culture, Cell death, Cell membranes, DNA, DNA damages, Electrophoresis, Endothelial cells, Few-layer graphene, Graphene, Medical applications, Microarrays, Mitochondria, Mitochondrial superoxide, oxidative stress, Oxygen, Primary cells, Single cell gel electrophoresis, toxicity, Umbilical vein endothelial cells

Abstract:

<p>Despite graphene being proposed for a multitude of biomedical applications, there is a dearth in the fundamental cellular and molecular level understanding of how few-layer graphene (FLG) interacts with human primary cells. Herein, using human primary umbilical vein endothelial cells as model of vascular transport, we investigated the basic mechanism underlying the biological behavior of graphene. Mechanistic toxicity studies using a battery of cell based assays revealed an organized oxidative stress paradigm involving cytosolic reactive oxygen stress, mitochondrial superoxide generation, lipid peroxidation, glutathione oxidation, mitochondrial membrane depolarization, enhanced calcium efflux, all leading to cell death by apoptosis/necrosis. We further investigated the effect of graphene interactions using cDNA microarray analysis and identified potential adverse effects by down regulating key genes involved in DNA damage response and repair mechanisms. Single cell gel electrophoresis assay/Comet assay confirmed the DNA damaging potential of graphene towards human primary cells. © 2016 Elsevier Inc.</p>

Notes:

cited By 0

Cite this Research Publication

Aab Sasidharan, Swaroop, Sa, Chandran, Pab, Shantikumar V Nair, and Dr. Manzoor K., “Cellular and molecular mechanistic insight into the DNA-damaging potential of few-layer graphene in human primary endothelial cells”, Nanomedicine: Nanotechnology, Biology, and Medicine, vol. 12, pp. 1347-1355, 2016.

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