Chitosan-hyaluronic acid/nano silver composite sponges for drug resistant bacteria infected diabetic wounds

URL:

http://www.scopus.com/inward/record.url?eid=2-s2.0-84885074193&partnerID=40&md5=e4391cf97dba2656c6194f9276d7abf9

Keywords:

antibacterial activity, antibiotic resistance, antimicrobial activity, article, biodegradation, cell adhesion, chitosan, concentration (parameters), controlled study, cytotoxicity, diabetes mellitus, diabetic foot, Escherichia coli, fibroblast, foot ulcer, freeze drying, human, human cell, hyaluronic acid, infrared spectroscopy, Klebsiella pneumoniae, methicillin resistant Staphylococcus aureus, nano silver composite sponge, nanocomposite, nonhuman, porosity, Pseudomonas aeruginosa, scanning electron microscopy, silver nanoparticle, Staphylococcus aureus, wound dressing, wound infection

Abstract:

The aim of this work was to develop an antimicrobial sponge composed of chitosan, hyaluronic acid (HA) and nano silver (nAg) as a wound dressing for diabetic foot ulcers (DFU) infected with drug resistant bacteria. nAg (5-20. nm) was prepared and characterized. The nanocomposite sponges were prepared by homogenous mixing of chitosan, HA and nAg followed by freeze drying to obtain a flexible and porous structure. The prepared sponges were characterized using SEM and FT-IR. The porosity, swelling, biodegradation and haemostatic potential of the sponges were also studied. Antibacterial activity of the prepared sponges was analysed using Escherichia coli, Staphylococcus aureus, methicillin-resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa and Klebsiella pneumonia. Chitosan-HA/nAg composite sponges showed potent antimicrobial property against the tested organisms. Sponges containing higher nAg (0.005%, 0.01% and 0.02%) concentrations showed antibacterial activity against MRSA. Cytotoxicity and cell attachment studies were done using human dermal fibroblast cells. The nanocomposite sponges showed a nAg concentration dependent toxicity towards fibroblast cells. Our results suggest that this nanocomposite sponges could be used as a potential material for wound dressing for DFU infected with antibiotic resistant bacteria if the optimal concentration of nAg exhibiting antibacterial action with least toxicity towards mammalian cells is identified. © 2013 Elsevier B.V.

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