Publication Type:

Journal Article

Source:

Mitochondrion, Volume 40, p.58-70 (2018)

Abstract:

<p>Cellular transformation owing to cigarette smoking is due to chronic exposure and not acute. However, systematic studies to understand the molecular alterations in lung cells due to cigarette smoke are lacking. To understand these molecular alterations induced by chronic cigarette smoke exposure, we carried out tandem mass tag (TMT) based temporal proteomic profiling of lung cells exposed to cigarette smoke for upto 12months. We identified 2620 proteins in total, of which 671 proteins were differentially expressed (1.5-fold) after 12months of exposure. Prolonged exposure of lung cells to smoke for 12months revealed dysregulation of oxidative phosphorylation and overexpression of enzymes involved in TCA cycle. In addition, we also observed overexpression of enzymes involved in glutamine metabolism, fatty acid degradation and lactate synthesis. This could possibly explain the availability of alternative source of carbon to TCA cycle apart from glycolytic pyruvate. Our data indicates that chronic exposure to cigarette smoke induces mitochondrial metabolic reprogramming in cells to support growth and survival.</p>

Cite this Research Publication

H. S. Solanki, Babu, N., Jain, A. P., Bhat, M. Younis, Puttamallesh, V. N., Advani, J., Raja, R., Mangalaparthi, K. K., Kumar, M. M., Prasad, T. S. Keshava, Mathur, P. Prakash, Sidransky, D., Gowda, H., and Chatterjee, A., “Cigarette smoke induces mitochondrial metabolic reprogramming in lung cells.”, Mitochondrion, vol. 40, pp. 58-70, 2018.

207
PROGRAMS
OFFERED
6
AMRITA
CAMPUSES
15
CONSTITUENT
SCHOOLS
A
GRADE BY
NAAC, MHRD
8th
RANK(INDIA):
NIRF 2018
150+
INTERNATIONAL
PARTNERS
  • Amrita on Social Media

  • Contact us

    Amrita Vishwa Vidyapeetham,
    Amritanagar,
    Coimbatore - 641 112,
    Tamil Nadu, India.
    • Fax                 : +91 (422) 268 6274
    • Coimbatore   : +91 (422) 268 5000
    • Amritapuri    : +91 (476) 280 1280
    • Bengaluru     : +91 (080) 251 83700
    • Kochi              : +91 (484) 280 1234
    • Mysuru          : +91 (821) 234 3479
    • Chennai         : +91 (44 ) 276 02165
    • Contact Details »