Publication Type:

Journal Article

Source:

Eur J Pharm Sci, Volume 96, p.193-206 (2017)

URL:

http://www.sciencedirect.com/science/article/pii/S0928098716303591

Keywords:

Administration, Cutaneous, Animals, Anti-Inflammatory Agents, Cell Line, chitin, Clobetasol, Drug Carriers, Humans, Mice, Mice, Inbred BALB C, Organ Culture Techniques, Polyethylene glycols, polyethyleneimine, psoriasis, Skin Absorption, Skin Cream, Swine

Abstract:

<p>In the present study chitin nanogel loaded with anti-psoriatic drug clobetasol was developed (CLCNG) for its topical delivery in psoriasis. CLCNG had the particle size of 132±14nm, with gel like consistency, stability in refrigerator, having higher drug release properties at acidic pH. CLCNG exhibited significant toxicity towards HaCaT and THP-1cell lines by MTT assay. The uptake of nanogel by HaCaT cell lines was confirmed by fluorescent microscopy. CLCNG at 0.35mg/ml exhibited significant anti-inflammatory activity with an average of 65% and 70% inhibition in COX and LOX activities expressed in THP-1 cells. In vitro skin permeation studies revealed the increased transdermal flux with fragmented stratum corneum and loosened epidermal layers in CLCNG treated samples, compared with control drug solution. The in vivo anti-psoriatic studies done on imiquimod model confirmed the potential benefits of the nanogel for the topical delivery of clobetasol in psoriasis.</p>

Cite this Research Publication

R. Panonnummal, Dr. Jayakumar Rangasamy, and Sabitha, M., “Comparative Anti-psoriatic Efficacy Studies of Clobetasol Loaded Chitin Nanogel and Marketed Cream”, Eur J Pharm Sci, vol. 96, pp. 193-206, 2017.