Publication Type:

Journal Article

Source:

Journal of Colloid and Interface Science, Volume 360, Number 1, p.39-51 (2011)

URL:

http://www.scopus.com/inward/record.url?eid=2-s2.0-79957875621&partnerID=40&md5=4c05eaf24318bc3973b3d9c0c52fbe75

Keywords:

animal cell, Animals, Antineoplastic Agents, Antitumor, apoptosis, article, biocompatibility, Biocompatible Materials, Biodegradable polymers, cancer cell culture, Cancer drug delivery, Caprolactam, Cell culture, Cell death, Cell Survival, cellular distribution, chitosan, chitosan g poly(n vinylcaprolactam), controlled study, Cultured, curcumin, cytotoxicity, Diseases, Drug delivery, drug delivery system, Drug Delivery Systems, drug formulation, drug release, Drug Screening Assays, drug uptake, flow cytometry, Fluorescence, Fourier Transform Infrared, human, human cell, Humans, Hydrogels, in vitro study, LCST, Mice, Molecular Structure, mouse, nanoparticle, Nanoparticles, neoplasm, nonhuman, particle size, polymer, priority journal, Specific toxicity, Spectroscopy, Stereoisomerism, Structure-Activity Relationship, Surface properties, temperature, Thermo-responsive, toxicity, Tumor Cells, ultraviolet spectrophotometry, unclassified drug

Abstract:

This study aims at the formulation of curcumin with biodegradable thermoresponsive chitosan-g-poly (N-vinylcaprolactam) nanoparticles (TRC-NPs) for cancer drug delivery. The spherical curcumin-loaded nanoparticles of size 220. nm were characterized, and the biological properties were studied using flow cytometry and cytotoxicity by MTT assay. The in vitro drug release was higher at above LCST compared to that at below LCST. TRC-NPs in the concentration range of 100-1000 μg/mL were non-toxic to an array of cell lines. The cellular localization of the curcumin-loaded TRC-NPs was confirmed from green fluorescence inside the cells. The time-dependent curcumin uptake by the cells was quantified by UV spectrophotometer. Curcumin-loaded TRC-NPs showed specific toxicity to cancer cells at above their LCST. Flow cytometric analysis showed increased apoptosis on PC3 compared to L929 by curcumin-loaded TRC-NPs. These results indicate that novel curcumin-loaded TRC-NPs could be a promising candidate for cancer drug delivery. © 2011 Elsevier Inc.

Notes:

cited By 51

Cite this Research Publication

S. N. Rejinold, Muthunarayanan, M., Divyarani, V. V., Sreerekha, P. R., Chennazhi, K. P., Nair, S. V., Tamura, H., and Jayakumar, R., “Curcumin-loaded biocompatible thermoresponsive polymeric nanoparticles for cancer drug delivery”, Journal of Colloid and Interface Science, vol. 360, pp. 39-51, 2011.