Publication Type:

Journal Article


Immunological Investigations, Informa Healthcare, Volume 24, Number 4, p.565-571 (1995)



adult, article, Carcinoma, Cell Transformation, clinical article, controlled study, Cultured, deoxyadenosine triphosphate, Disease Progression, female, gene expression, Gene Expression Regulation, guanidine isothiocyanate, human, human cell, Immunologic Deficiency Syndromes, interleukin 2, interleukin 2 receptor, Interleukin-2, isothiocyanic acid derivative, Lymphocyte Activation, male, Messenger, messenger RNA, neoplasm, Neoplasm Proteins, Neoplastic, Non-U.S. Gov't, northern blotting, phosphorus 32, phytohemagglutinin, Phytohemagglutinins, priority journal, receptor subtype, Receptors, RNA, stomach carcinoma, Stomach Neoplasms, Support, T lymphocyte activation, T-Lymphocytes, Tumor Cells, unclassified drug


Peripheral blood T lymphocytes from 15 patients with gastric carcinoma and 6 normal healthy controls were evaluated for Interleukin-2 R gene expression. Total RNA was extracted from T cell-enriched fractions of patients as well as from control peripheral blood lymphocytes, with or without mitogenic stimulation. The presence of mRNA for IL-2 R alpha evaluated by Northern blot analysis revealed that unstimulated T cells expressed lower levels of IL-2 R mRNA than PHA stimulated T cells. Expression of both IL-2 R alpha transcripts (3.5 and 1.5 Kb) were either not detectable or only weakly detectable on T lymphocytes from patients even after mitogenic stimulation. In contrast, a significant rise in the expression of both IL-2 R alpha transcripts was observed on T cells from normal controls followed by mitogenic challenge. This paper reports on the defective IL-2 R alpha gene expression in gastric carcinoma patients, which may explain one of the causes of immunodeficiency associated with neoplastic transformation and progression. © 1995 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.


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Cite this Research Publication

K. Suresh, Vasudevan, D. M., and Jayavardhanan, K. K., “Defective interleukin - 2 r gene expression in gastric carcinoma patients”, Immunological Investigations, vol. 24, pp. 565-571, 1995.