Publication Type:

Journal Article

Source:

International Journal of Biological Macromolecules, Volume 74, p.36 - 43 (2015)

URL:

http://www.sciencedirect.com/science/article/pii/S0141813014007545

Keywords:

Polymorphonuclear leukocytes-Staphylococcus aureus

Abstract:

Abstract Polymorphonuclear leukocytes (PMNs) provide the primary host defence against invading pathogens by producing reactive oxygen species (ROS) and microbicidal products. However, few pathogens can survive for a prolonged period of time within the PMNs. Additionally their intracellular lifestyle within the \{PMNs\} protect themselves from the additional lethal action of host immune systems such as antibodies and complements. Antibiotic delivery into the intracellular compartments of \{PMNs\} is a major challenge in the field of infectious diseases. In order to deliver antibiotics within the \{PMNs\} and for the better treatment of intracellular bacterial infections we synthesized rifampicin (RIF) loaded amorphous chitin nanoparticles (RIF-ACNPs) of 350 ± 50 nm in diameter. RIF-ACNPs nanoparticles are found to be non-hemolytic and non-toxic against a variety of host cells. The release of rifampicin from the prepared nanoparticles was ∼60% in 24 h, followed by a sustained pattern till 72 h. The RIF-ACNPs nanoparticles showed 5–6 fold enhanced delivery of \{RIF\} into the intracellular compartments of PMNs. The RIF-ACNPs showed anti-microbial activity against Escherichia coli, Staphylococcus aureus and a variety of other bacteria. In summary, our results suggest that RIF-ACNPs could be used to treat a variety of intracellular bacterial infections.

Cite this Research Publication

K. T. Smitha, Nisha, N., Maya, S., Dr. Raja Biswas, and Dr. Jayakumar Rangasamy, “Delivery of rifampicin-chitin nanoparticles into the intracellular compartment of polymorphonuclear leukocytes”, International Journal of Biological Macromolecules, vol. 74, pp. 36 - 43, 2015.

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