Publication Type:

Journal Article


Nanotechnology, Volume 20, Number 19 (2009)



adsorption, antibody, article, Bipyramids, bone development, Bottom-up methods, cell adhesion, Cell membranes, Cell response, controlled study, cost effectiveness analysis, Cost effectives, cytotoxicity, Focal adhesions, human, human cell, Hydrothermal conditions, Hydrothermally, immunofluorescence, Implant materials, intracellular signaling, Mesoporous, molecular scaffold, Nano-structured surfaces, nanomaterial, nanorod, Nanoscaffolds, Nanostructures, Orthopedic implants, osteoblast, Osteoblast response, Osteoblastic cells, Osteoblasts, priority journal, Processing techniques, Protein adsorptions, Reaction mediums, Solution chemistries, Surface topography, Surface-Modified, Surfaces, Time durations, Titanium, Titanium implants, unclassified drug, vinculin, vinculin antibody


We report an interesting cell response to novel nanostructures formed on a titanium (Ti) surface by a simple non-lithographic bottom-up method. The surface topography of bio-implant materials dramatically influences their cell response. The aim of this study was to modify the surface of a titanium implant by a simple and cost effective processing technique and to determine its suitability for osteoblast attachment. A set of unique structures ranging from mesoporous nanoscaffolds, nanoflowers, nanoneedles, nanorods and octahedral bipyramids were fabricated by systematically tuning the hydrothermal conditions such as reaction medium composition, concentration, temperature and time duration. The cytotoxicity of surface modified Ti was assessed using human primary osteoblastic cells, and more than 90% of the cells were found to be viable after 24 h of incubation. Protein adsorption studies revealed that the surface modified nanostructures on titanium adsorbed more proteins, suggesting that they are capable of promoting cell adhesion/attachment. Immunofluorescence studies with vinculin antibody identified a distinctly different spread pattern of osteoblastic cells on hydrothermally modified nanostructured surfaces, indicating the formation of the focal adhesion points required for intracellular signaling. Thus, based on our results, we suggest that this study may present one of the best designs and systematic syntheses of biocompatible nanostructures on metallic Ti for orthopedic implant applications. © 2009 IOP Publishing Ltd.


cited By 14

Cite this Research Publication

V. V. D. Rani, Manzoor, K., Menon, D., Selvamurugan, N., and Nair, S. V., “The design of novel nanostructures on titanium by solution chemistry for an improved osteoblast response”, Nanotechnology, vol. 20, 2009.