A simple and sensitive visible spectrophotometric method has been developed for the determination of Tiaprofenic acid from its bulk drug and formulations. This method involves charge-transfer complex formation of tiaprofenic acid with in situ oxidized form haemaetin obtained from haematoxylin and chloramin-T (CAT) in the presence of pH 7 buffer solution, to form purple colored species of maximum wavelength at 555 nm. Regression analysis of Beer's law plot showed good correlation in the concentration range of 16-80μg/ml. The proposed method is applied to commercial available tablets and the results are statistically compared with those obtained by the UV reference method and validated by recovery studies. The results are found satisfactory and reproducible. The method is applied successfully for the estimation of the tiaprofenic acid in formulations without the interference of excipients. The method offers the advantages of rapidity, simplicity and sensitivity and can be easily applied to resource-poor settings without the need for expensive instrumentation and reagents.
K. Raghubabu, Dr. Madhu Mohan N., Kalyanaramu, B., Ramdas, C., and Rao, M. N., “Development of new visible specrophotometric determination of tiaprofenic Acid in bulk and formulations”, Asian J Pharm Clin Res, vol. 6, pp. 77–79, 2013.