Publication Type:

Journal Article


Chemical Communications, Royal Society of Chemistry, Volume 55, Number 45, p.6429-6432 (2019)



2, 3T3 cell line, 3T3 Cells, 4 dinitrophenol, 4-Dinitrophenol, animal, Animals, cell proliferation, chemical structure, chemistry, drug effect, Fluorescence, fluorescence imaging, fluorescent dye, Fluorescent Dyes, HCT 116 cell line, HCT116 Cells, HeLa cell line, Hela Cells, human, Humans, Hydrogen sulfide, Membrane Potential, metabolism, Mice, Mitochondria, Mitochondrial, mitochondrial membrane potential, mitochondrion, Molecular Structure, mouse, Optical imaging, reactive oxygen metabolite, Reactive Oxygen Species, spectrofluorometry, Spectrometry


Concurrently, manipulation of mitochondrial activity and its monitoring have enormous significance in cancer therapy and diagnosis. In this context, a fluorescent probe MitoDP has been developed for validating H2S mediated protonophore (2,4-dinitrophenol, DNP) induced mitochondrial membrane potential change, ROS formation and ATP depletion in cancer cells. The extent of protonophore activation for mitochondrial dysfunction is monitored through fluorescence signalling at 450 nm. The current study provides a proof for the concept of endogenous H2S-mediated controlled and spatial release of bioactive agents, or toxins specifically in mitochondria of cancer cells. © 2019 The Royal Society of Chemistry.


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Cite this Research Publication

K. N. Bobba, Binoy, A., Koo, S., Nedungadi, D., Podder, A., Sharma, A., Mishra, N., Kim, J. S., and Bhuniya, S., “Direct readout protonophore induced selective uncoupling and dysfunction of individual mitochondria within cancer cells”, Chemical Communications, vol. 55, pp. 6429-6432, 2019.