Publication Type:

Journal Article


International Journal of Biological Macromolecules, Volume 51, Number 4, p.392-399 (2012)



Anti-Bacterial Agents, antibacterial activity, article, Bacteria (microorganisms), bacterial cell, bacterial growth, biocompatibility, Biocompatible Materials, Capsules, carboxymethyl chitosan nanoparticle, cell viability, chitosan, concentration response, controlled study, Drug Carriers, drug cytotoxicity, drug delivery system, drug efficacy, drug release, drug targeting, encapsulation, endocytosis, gelation, Gels, HEK293 Cells, Hemolysis, human, human cell, Humans, hydrophilicity, intracellular killing, Intracellular Space, macrophage, nanoparticle, Nanoparticles, nonhuman, particle size, Platelet activation, Staphylococcus aureus, tetracycline, thrombocyte activation, unclassified drug, zeta potential


Intracellular bacterial infections are recurrent, persistent and are difficult to treat because of poor penetration and limited availability of antibiotics within macrophages and epithelial cells. We developed biocompatible, 200. nm sized tetracycline encapsulated O-carboxymethyl chitosan nanoparticles (Tet-O-CMC Nps) via ionic gelation for its sustained delivery of Tet into cells. S. aureus binds and aggregates with Tet-O-CMC Nps increasing drug concentrations at the infection site. Tet-O-CMC Nps were sixfold more effective in killing intracellular S. aureus compared to Tet alone in HEK-293 and differentiated THP1 macrophage cells proving it to be an efficient nanomedicine to treat intracellular S. aureus infections. © 2012 Elsevier B.V.


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Cite this Research Publication

S. Maya, Indulekha, S., Sukhithasri, V., Smitha, K. T., Nair, S. V., Jayakumar, R., and R.a Biswas, “Efficacy of tetracycline encapsulated O-carboxymethyl chitosan nanoparticles against intracellular infections of Staphylococcus aureus”, International Journal of Biological Macromolecules, vol. 51, pp. 392-399, 2012.