Publication Type:

Journal Article

Source:

Carbohydrate Polymers, Volume 83, Number 2, p.452-461 (2011)

URL:

http://www.scopus.com/inward/record.url?eid=2-s2.0-78349312050&partnerID=40&md5=c5a213ba840ca2fd31034cf19a5ebc8b

Keywords:

AFM, Biochemistry, Biological properties, Biomedical applications, Cancer cells, Carboxymethyl chitosan, Cellular uptake, Chemotherapeutic agents, chitin, chitosan, curcumin, cytotoxicity, Drug delivery, Drug delivery applications, fluorescence microscopy, In-vitro, Mean diameter, MTT assays, Nanocarriers, Nanoformulation, Nanoparticles, O-carboxymethyl chitosans, Particle size analysis, SEM, Size analysis, Spherical particle, Toxic materials, Watersoluble, XRD

Abstract:

Carboxymethyl chitosan derivatives are widely used for biomedical applications because of their non-toxic and biodegradable properties. Curcumin is a phytochemical with immense biological properties. But its hydrophobicity and poor oral bioavailability limits its application as a chemotherapeutic agent. To increase the oral bioavailability, we developed curcumin loaded O-CMC nanoparticles (curcumin-O-CMC Nps). The prepared nanoparticles were characterized by DLS, AFM, SEM, FT-IR, XRD and TG/DTA. Size analysis studies revealed spherical particles with mean diameter of about 150 ± 30 nm. Curcumin was entrapped in O-CMC with an efficiency of 87%. In vitro drug release profile was studied at 37 °C under different pHs (7.4 and 4.5) with and without lysozyme. Cytotoxicity studies by MTT assay indicated that curcumin-O-CMC Nps were toxic to cancer and non-toxic to normal cells. Cellular uptake of the curcumin-O-CMC Nps was analyzed by fluorescence microscopy and FACS. Overall these studies indicated O-CMC as a promising nanomatrix for drug delivery applications. © 2010 Elsevier Ltd. All rights reserved.

Notes:

cited By 75

Cite this Research Publication

A. Anitha, Maya, S., Deepa, N., Chennazhi, K. P., Nair, S. V., Tamura, H., and Jayakumar, R., “Efficient water soluble O-carboxymethyl chitosan nanocarrier for the delivery of curcumin to cancer cells”, Carbohydrate Polymers, vol. 83, pp. 452-461, 2011.