For the first time reporting a pencil graphite electrode modified with gold nanoparticles and non-conducting polymeric film of o-aminophenol for the selective determination of pyridoxine (VB6) from other water-soluble vitamins in human blood. The fabricated electrode was characterized by scanning electron microscopy, energy-dispersive X-ray spectroscopy, electrochemical impedance spectroscopy, and attenuated total reflection infrared spectroscopy. Voltammetric response of VB6 on the fabricated electrode was investigated out using cyclic and differential pulse voltammetric techniques. Under optimized conditions, an irreversible oxidation peak at + 0.632 V was observed which corresponds to the oxidation of VB6. The oxidation peak current and the pyridoxine concentration were found to be linear in the range from 5 to 200 μM with a detection limit of 0.30 μM. The sensitivity of the proposed electrode was calculated to be 3.373 μA/μM/cm2 with excelled reproducibility and stability. The electrode is suitable for the routine analysis of VB6 in human blood samples as it highly selective from other water-soluble vitamins.
R. Rejithamol and Dr. Beena S., “Electrochemical quantification of pyridoxine (VB6) in human blood from other water-soluble vitamins”, Chemical Papers, vol. 74, no. 6, pp. 2011 - 2020, 2020.