Publication Type:

Journal Article


Tissue Engineering - Part A, Mary Ann Liebert Inc., Volume 18, Number 17-18, p.1867-1881 (2012)



adsorption, alkaline phosphatase, article, Atomic, atomic absorption spectrometry, Bioactivity, biocompatibility, Biomechanics, Bone, Bone and Bones, bone development, bone mineralization, Bone tissue engineering, Bone tissue regeneration, Calcification, cell adhesion, Cell culture, Cell death, cell differentiation, cell motion, Cell Movement, cell proliferation, cell shape, Cell Survival, chemistry, Cytology, drug effect, enzymology, Fetal Blood, fetus blood, Fiber surface, flow cytometry, Fluorescence, fluorescence microscopy, Fourier Transform Infrared, human, Human mesenchymal stem cells (hMSCs), Humans, infrared spectroscopy, Materials Testing, Mechanical properties, mesenchymal stem cell, mesenchymal stroma cell, Mesenchymal Stromal Cells, metabolism, methodology, microscopy, Nano scale, nanofiber, Nanofibers, Nanofibrous scaffolds, nanoparticle, Nanoparticles, Osteogenesis, Osteogenic differentiation, Osteogenic materials, Osteogenic potential, PCL nanofibers, Physiologic


Poly(caprolactone) (PCL) has been frequently considered for bone tissue engineering because of its excellent biocompatibility. A drawback, however, of PCL is its inadequate mechanical strength for bone tissue engineering and its inadequate bioactivity to promote bone tissue regeneration from mesenchymal stem cells. To correct this deficiency, this work investigates the addition of nanoparticles of silica (nSiO2) to the scaffold to take advantage of the known bioactivity of silica as an osteogenic material and also to improve the mechanical properties through nanoscale reinforcement of the PCL fibers. The nanocomposite scaffolds and the pristine PCL scaffolds were evaluated physicochemically, mechanically, and biologically in the presence of human mesenchymal stem cells (hMSCs). The results indicated that, when the nanoparticles of size approximately 10 nm (concentrations of 0.5% and 1% w/v) were embedded within, or attached to, the PCL nanofibers, there was a substantial increase in scaffold strength, protein adsorption, and osteogenic differentiation of hMSCs. These nSiO2 nanoparticles, when directly added to the cells evidently pointed to ingestion of these particles by the cells followed by cell death. The polymer nanofibers appeared to protect the cells by preventing ingestion of the silica nanoparticles, while at the same time adequately exposing them on fiber surfaces for their desired bioactivity. © Copyright 2012, Mary Ann Liebert, Inc.


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Cite this Research Publication

N. Ganesh, Jayakumar, R., Koyakutty, M., Ullas Mony, and Nair, S. V., “Embedded silica nanoparticles in poly(caprolactone) nanofibrous scaffolds enhanced osteogenic potential for bone tissue engineering”, Tissue Engineering - Part A, vol. 18, pp. 1867-1881, 2012.