Publication Type:

Journal Article


International Journal of Biological Macromolecules, Volume 55, p.289-294 (2013)



absorption, Alginates, alginic acid, animal cell, Animals, article, Biocompatible Materials, Bone regeneration, cell encapsulation, Cell Line, cell proliferation, Cell Survival, cell transplantation, cell viability, composite material, controlled study, covalent bond, cross linking, Glucuronic Acid, Hexuronic Acids, horseradish peroxidase, hyaluronic acid, hyaluronidase, hydrogel, Hydrogels, hydrogen peroxide, Materials Testing, Mice, mouse, nonhuman, oxidative coupling, tissue engineering, tyramine, Water, water absorption, water transport


An injectable composite gel was developed from alginic and hyaluronic acid. The enzymatically cross-linked injectable gels were prepared via the oxidative coupling of tyramine modified sodium algiante and sodium hyaluronate in the presence of horse radish peroxidase (HRP) and hydrogen peroxide (H2O2). The composite gels were prepared by mixing equal parts of the two tyraminated polymer solutions in 10U HRP and treating with 1.0% H2O2. The properties of the alginate gels were significantly affected by the addition of hyaluronic acid. The percentage water absorption and storage modulus of the composite gels were found to be lower than the alginate gels. The alginate and composite gels showed lower protein release compared to hyaluronate gels in the absence of hyaluronidase. Even hyaluronate gels showed only approximately 10% protein release after 14 days incubation in phosphate buffer solution. ATDC-5 cells encapsulated in the injectable gels showed high cell viability. The composite gels showed the presence of enlarged spherical cells with significantly higher metabolic activity compared to cells in hyaluronic and alginic acid gels. The results suggest the potential of the composite approach to develop covalently cross-linked hydrogels with tuneable physical, mechanical, and biological properties. © 2013 Elsevier B.V.


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Cite this Research Publication

, “Enzymatically cross-linked alginic-hyaluronic acid composite hydrogels as cell delivery vehicles”, International Journal of Biological Macromolecules, vol. 55, pp. 289-294, 2013.