Publication Type:

Journal Article


Methods in Neurosci, Issue 29, p.319-343 (1996)


Publisher Summary This chapter presents a number of experimental approaches that have proved to be very useful in elucidating the mechanism(s) involved in epidermal growth factor (EGF) -mediated stimulation of cardiac adenylylcyclase. However, there are several additional questions pertaining to the detailed understanding of the mechanism(s) involved in interactions between the \{EGF\} receptor and Gs interaction. Thus, the in vitro studies demonstrate the juxtamembrane region of the \{EGF\} receptor that is important for stimulation of Gs by performing mutation(s) of key residue(s) in the juxtamembrane region of the \{EGF\} receptor, to determine whether the ability of \{EGF\} to stimulate adenylylcyclase can be obliterated. Similarly, it is important to determine the region(s) of Gsα that interact with the \{EGF\} receptor. It is also determine that what elements confer specificity to the ability of \{EGF\} to stimulate adenylylcyclase. The latter goal poses a particularly important question, because \{EGF\} does not elevate cAMP content in all cells that express the \{EGF\} receptor, Gs, and adenylylcyclase.

Cite this Research Publication

T. B. Patel, H .Sun, H. Poppleton, Dr. Bipin G. Nair, H. M. Rashed, and Y. Yu, “Epidermal growth factor mediated regulation of G-proteins and adenylyl cyclase in cardiac muscle”, Methods in Neurosci, no. 29, pp. 319-343, 1996.