Publication Type:

Journal Article

Source:

International Journal of Biological Macromolecules, Volume 48, Number 2, p.336-344 (2011)

URL:

http://www.scopus.com/inward/record.url?eid=2-s2.0-79551686584&partnerID=40&md5=d52c772b0fdfa940c40b544fac3d7f06

Keywords:

3 (4, 5 dimethyl 2 thiazolyl) 2, 5 diphenyltetrazolium bromide, Animals, article, biocompatibility, biomineralization, bone defect, cell adhesion, Cell death, Cell Line, cell viability, chitin, chitosan, controlled study, fibroblast, Fibroblasts, Fourier Transform Infrared, freeze drying, human, human cell, human tissue, Humans, in vitro study, infrared spectroscopy, mesenchymal stem cell, Mice, Minerals, molecular weight, nanocomposite, Nanostructures, osteoblast, scanning electron microscopy, Spectroscopy, tissue engineering, tissue scaffold, Tissue Scaffolds, Titanium, Titanium dioxide, X ray diffraction, X-Ray Diffraction

Abstract:

In this study, we prepared chitin-chitosan/nano TiO2 composite scaffolds using lyophilization technique for bone tissue engineering. The prepared composite scaffold was characterized using SEM, XRD, FTIR and TGA. In addition, swelling, degradation and biomineralization capability of the composite scaffolds were evaluated. The developed composite scaffold showed controlled swelling and degradation when compared to the control scaffold. Cytocompatibility of the scaffold was assessed by MTT assay and cell attachment studies using osteoblast-like cells (MG-63), fibroblast cells (L929) and human mesenchymal stem cells (hMSCs). Results indicated no sign of toxicity and cells were found attached to the pore walls within the scaffolds. These results suggested that the developed composite scaffold possess the prerequisites for tissue engineering scaffolds and it can be used for tissue engineering applications. © 2010 Elsevier B.V.

Notes:

cited By 33

Cite this Research Publication

R. Jayakumar, Ramachandran, R., Divyarani, V. V., Chennazhi, K. P., Tamura, H., and Nair, S. V., “Fabrication of chitin-chitosan/nano TiO2-composite scaffolds for tissue engineering applications”, International Journal of Biological Macromolecules, vol. 48, pp. 336-344, 2011.