Publication Type:

Journal Article


International Journal of Biological Macromolecules, Volume 48, Number 4, p.571-576 (2011)



4', 6 diamidino 2 phenylindole, adsorption kinetics, animal cell, Animals, article, biological activity, biomineralization, Bone and Bones, cell adhesion, Cell Line, cell proliferation, cell spreading, cell viability, chitosan, Contact angle, controlled study, cytotoxicity test, Electron, Electrospinning, embryo, fibroblast, fibrosarcoma, human, human cell, Humans, hydrophilicity, hydrophobicity, in vitro study, Mice, microscopy, molecular scaffold, mouse, Murinae, nanofabrication, Nanofibers, NIH 3T3 Cells, nonhuman, osteosarcoma cell, polycaprolactone, Polyesters, Scanning, scanning electron microscopy, skin, tissue engineering, Tissue Scaffolds, Tumor


Chitosan/poly(caprolactone) (CS/PCL) nanofibrous scaffold was prepared by a single step electrospinning technique. The presence of CS in CS/PCL scaffold aided a significant improvement in the hydrophilicity of the scaffold as confirmed by a decrease in contact angle, which thereby enhanced bioactivity and protein adsorption on the scaffold. The cyto-compatibility of the CS/PCL scaffold was examined using human osteoscarcoma cells (MG63) and found to be non toxic. Moreover, CS/PCL scaffold was found to support the attachment and proliferation of various cell lines such as mouse embryo fibroblasts (NIH3T3), murine aneuploid fibro sarcoma (L929), and MG63 cells. Cell attachment and proliferation was further confirmed by nuclear staining using 4′,6-diamidino-2-phenylindole (DAPI). All these results indicate that CS/PCL nanofibrous scaffold would be an excellent system for bone and skin tissue engineering. © 2011 Elsevier B.V.


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Cite this Research Publication

K. T. Shalumon, Anulekha, K. H., Chennazhi, K. P., Tamura, H., Nair, S. V., and Dr. Jayakumar Rangasamy, “Fabrication of Chitosan/poly(caprolactone) Nanofibrous Scaffold for Bone and Skin Tissue Engineering”, International Journal of Biological Macromolecules, vol. 48, pp. 571-576, 2011.