Publication Type:

Journal Article


Journal of Biomedical Nanotechnology, Volume 9, Number 5, p.790-800 (2013)



Applications, article, Biocompatible, Biomechanics, cell adhesion, Cell Culture Techniques, cell interaction, cell proliferation, cell spreading, cell viability, Cells, chemical analysis, chemical composition, Coated Materials, Contact angle, Cultured, Electroplating, Electrospinning, Fabrication, Fabrication and characterizations, Fetal Blood, Fibrin, human, human cell, Human mesenchymal stem cells, Humans, infant, Leukocytes, Materials Testing, Mechanical properties, mesenchymal stem cell, Mesenchymal Stromal Cells, Microtechnology, Mononuclear, Multiscales, nanofabrication, nanofiber, Nanofibers, Native extracellular matrix, newborn, particle size, phosphotungstic acid, physical chemistry, Physico-chemical characterization, Poly (vinyl alcohol) (PVA), polycaprolactone, Polyesters, Scaffolds (biology), scanning electron microscopy, Stem cells, Tissue, tissue engineering, Tissue engineering applications, tissue scaffold, Tissue Scaffolds


Fabricating scaffolds mimicking the native extracellular matrix (ECM) in both structure and function is a key challenge in the field of tissue engineering. Previously we have demonstrated a novel electrospinnig method for the fabrication of fibrin nanofibers using Poly(vinyl alcohol) (PVA) as an 'electrospinning-driving' polymer. Here we demonstrate the fabrication and characterization of a multiscale fibrin based composite scaffold with polycaprolactone (PCL) by sequential electrospinning of PCL microfibers and fibrin nanofibers. This multiscale scaffold has great potential for tissue engineering applications due to the combined benefits of biological nanofibers such as cell attachment and proliferation and that of microfibers such as open structure, larger pore size and adequate mechanical strength. Physico chemical characterization of the electrospun scaffold was done by Scanning Electron Microscopy (SEM), Contact angle analysis, fibrin specific Phosphotungstic acid haematoxyllin (PTAH) staining and evaluation of mechanical properties. SEM data revealed the formation of bead free nanofibers of fibrin with a fiber diameter ranging from 50-500 nm and microfibers of PCL in the size range of 1 microns to 2.5 microns. These dimensions mimic the hierarchical structure of ECM found in native tissues. Cell attachment and viability studies using human mesenchymal stem cells (hMSC) revealed that the scaffold is non toxic and supports cell attachment, spreading and proliferation. In addition, we examined the inflammatory potential of the scaffold to demonstrate its usefulness in tissue engineering applications. Copyright © 2013 American Scientific Publishers All rights reserved.


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Cite this Research Publication

, “Fabrication of fibrin based electrospun multiscale composite scaffold for tissue engineering applications”, Journal of Biomedical Nanotechnology, vol. 9, pp. 790-800, 2013.