GC-MS, HPLC and AAS analysis of fatty acids, amino acids and minerals in red algae ampheroa anceps
Publication Type:Journal Article
Source:International Journal of Pharmacy and Pharmaceutical Sciences, Volume 4, Number SUPPL.1, p.187-190 (2012)
Keywords:alanine, Alkalinity, Amino Acid, Amphiroa anceps, arginine, article, aspartic acid, atomic absorption spectrometry, calcium, colorimetry, copper, cysteine, drug determination, drug hydrolysis, dry weight, fatty acid, gas chromatography, glutamic acid, glycine, high performance liquid chromatography, histidine, human, iron, isoleucine, leucine, lysine, magnesium, Manganese, methionine, mineral, phenylalanine, potassium, proline, red alga, serine, Sodium, thioglycolic acid, threonine, tryptophan, tyrosine, unindexed drug, valine
Fatty acid, amino acid, minerals, and trace metals of Ampheroa anceps were determined. Fatty acids are determined by gas chromatography-mass spectrometry (GC-MS), using flame ionization detector. In the case of fatty acids total 12 components were find out in which the major fatty acids encountered are 16:0, 20:4, 18:1n9, 18:2n6, and 14:0, etc. Amino acids are determined using high performance liquid chromatography (HPLC) and total 18 amino acids were found on the dry weight basis. Tryptophan was estimated colorimetrically following digestion under alkaline condition and estimated with thioglycolic acid reagent. Minerals identified by using Flame Photometry are sodium, potassium and calcium whereas iron, copper, Manganese, magnesium, etc using Atomic Absorption Spectroscopy.
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