Publication Type:

Journal Article

Source:

Free Radical Research, Taylor and Francis Ltd, Volume 49, Number 10, p.1173-1186 (2015)

URL:

http://www.scopus.com/inward/record.url?eid=2-s2.0-84940449497&partnerID=40&md5=a8fd164f2add6541f86b22c721cd1ac3

Keywords:

alanine aminotransferase, alanine aminotransferase blood level, alkaline phosphatase, alkaline phosphatase blood level, animal cell, animal experiment, animal model, animal tissue, antioxidant, article, aspartate aminotransferase, aspartate aminotransferase blood level, biochemical analysis, catalase, cobalt 60, comet assay, controlled study, DNA, DNA damage, drug bioavailability, drug effect, drug efficacy, drug screening, enzyme activation, enzyme activity, Enzyme inactivation, experimental hepatitis, experimental radiation injury, flow cytometry, gamma radiation, glutathione, gossypetin, histopathology, immunoblotting, immunofluorescence test, immunoglobulin enhancer binding protein, interleukin 6, ionizing radiation, lipid peroxidation, liver histology, liver toxicity, male, manganese superoxide dismutase, mitogen activated protein kinase kinase, mouse, Murinae, Mus, nonhuman, oxidative stress, phosphatidylinositol 3 kinase, protein c jun

Abstract:

Radioprotective action of gossypetin (GTIN) against gamma (γ)-radiation-induced oxidative stress in liver was explored in the present article. Our main aim was to evaluate the protective efficacy of GTIN against radiation-induced alteration of liver in murine system. To evaluate the effect of GTIN, it was orally administered to mice at a dose of 30 mg/kg body weight for three consecutive days prior to γ-radiation at a dose of 5 Gy. Radioprotective efficacy of GTIN were evaluated at physiological, cellular, and molecular level using biochemical analysis, comet assay, flow cytometry, histopathology, immunofluorescence, and immunoblotting techniques. Ionizing radiation was responsible for augmentation of hepatic oxidative stress in terms of lipid peroxidation and depletion of endogenous antioxidant enzymes. Immunoblotting and immunofluorescence studies showed that irradiation enhanced the nuclear translocation of nuclear factor kappa B (NF-B) level, which leads to hepatic inflammation. To investigate further, we found that radiation induced the activation of stress-activated protein kinase/c-Jun NH2-terminal kinase (SAPK/JNK)-mediated apoptotic pathway and deactivation of the NF-E2-related factor 2 (Nrf2)-mediated redox signaling pathway, whereas GTIN pretreatment ameliorated these radiation-mediated effects. This is the novel report where GTIN rationally validated the molecular mechanism in terms of the modulation of cellular signaling system instead of This is the novel report where GTIN is rationally validated in molecular terms to establish it as promising radioprotective agents. This might be fruitful especially for nuclear workers and defense personnel assuming the possibility of radiation exposure. © 2015 Informa UK, Ltd.

Notes:

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Cite this Research Publication

A. Khan, Manna, K., Das, D. K., Kesh, S. B., Sinha, M., Das, U., Biswas, S., Sengupta, A., Sikder, K., Datta, S., Ghosh, M., Chakrabarty, A., Banerji, A., and Dey, S., “Gossypetin ameliorates ionizing radiation-induced oxidative stress in mice liver-a molecular approach”, Free Radical Research, vol. 49, pp. 1173-1186, 2015.