The aim of the study was to assess the degree of liver toxicity produced by commonly used antibiotics using in vitro liver slices. Method: The freshly isolated liver lobes were transferred into KRH medium and sliced into small pieces using surgical blade. It was then preincubated in KRH medium at 37°C by replacing fresh medium every 10 minutes for 1 hour. Then the slices were incubated with different concentrations of sulfamethoxazole, clavulanic acid and tetracycline for 2 hours. After 2 hours the prepared liver homogenate was assayed for the markers like ALT, AST, and MDA level and catalase activity for the assessment of toxicity. Results: After incubation with the three antibiotics at different doses, the liver parameters were altered in a dose dependent fashion. At the highest concentration of the antibiotics (2000 µg/ml) the parameters were found to be elevated which shows a clear indication of the degree of toxicity. Conclusion: The observed data reveals that repeated dosing and consuming these antibiotics at higher doses may lead to hepatotoxicity and it can be indicated by change in the parameters.
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V. Simon, Sreerag, K. V., Sasikumar, R., and S.K. Kanthlal, “High dose antibiotics induced hepatotoxicity and altered markers: An in-vitro liver slices study”, Research Journal of Pharmacy and Technology, vol. 10, 2017.