Publication Type:

Journal Article

Source:

Journal of Biomedical Nanotechnology, Volume 9, Number 12, p.2117-2122 (2013)

URL:

http://www.scopus.com/inward/record.url?eid=2-s2.0-84888799209&partnerID=40&md5=fbc50ab258e394bf9fb20dcaa30f42e5

Keywords:

3-D scaffolds, article, Bone substitutes, Bone tissue engineering, Cell infiltration, Cell Movement, Cells, compression, concentration (parameters), confocal microscope, cryogrinding, Cultured, Cytology, Electron, Electrospinning, extracellular matrix, Fabrication, Fabrication technique, freeze drying, grinding, Humans, Lactic acid, Layer-by-layer methods, Layering techniques, microscopy, Microtechnology, Multiscale, nanofabrication, nanofiber, Nanofibers, Optimum concentration, Osseointegration, Osteoblasts, polylactic acid, Polymers, porosity, resin, Scaffolds (biology), Scanning, scanning electron microscopy, Three dimensional, Tissue, tissue engineering, tissue scaffold, Tissue Scaffolds

Abstract:

This work explains the fabrication and potential applicability of high thick three dimensional (3-D) electrospun multiscale fibrous scaffolds in tissue engineering by focusing on the possible fabrication techniques. Multiscale fibrous scaffold of poly(lactic acid) (PLA) was fabricated by combining nano and micro fibers in optimum concentrations. Finely chopped multiscale fibers were allowed to undergo compression, freeze-drying, resin embedding, cryo-grinding and layering techniques to make 3D scaffolds and the layer-by-layer method was found to be most suitable for 3-D scaffold fabrication. Cell studies in layered 3D scaffolds were performed using MG 63 cells and infiltration was observed using SEM and confocal microscope. Since the layered high thick 3D scaffold perfectly complies with the requirements, this could be proposed as one of the suitable methods for constructing 3D scaffolds for tissue engineering applications. Copyright © 2013 American Scientific Publishers. All rights reserved.

Notes:

cited By 2

Cite this Research Publication

, “High thick layer-by-layer 3D multiscale fibrous scaffolds for enhanced cell infiltration and it's potential in tissue engineering”, Journal of Biomedical Nanotechnology, vol. 9, pp. 2117-2122, 2013.