miRNAs regulate gene expression by binding to cognate mRNAs causing mRNA degradation or translational repression. Mass spectrometry-based proteomic analysis is being widely used to identify miRNA targets. The miR-200b miRNA cluster is often overexpressed in multiple cancer types, but the identity of the targets remains elusive. Using SILAC-based analysis, we examined the effects of overexpression of a miR-200b mimic or a control miRNA in fibrosarcoma cells. We identified around 300 potential targets of miR-200b based on a change in the expression of protein levels. We validated a subset of potential targets at the transcript level using quantitative PCR.
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Aa Marimuthu, Huang, T. - Cb, Selvan, L. D. Nac, Renuse, Sac, Nirujogi, R. Sad, Kumar, Pa, Pinto, S. Mae, Rajagopalan, Sf, Pandey, Abg h i, Harsha, H. Ca, and Chatterjee, Aa, “Identification of targets of miR-200b by a SILAC-based quantitative proteomic approach”, EuPA Open Proteomics, vol. 4, pp. 10-17, 2014.