Injectable alginate-O-carboxymethyl chitosan/nano fibrin composite hydrogels for adipose tissue engineering
Publication Type:Journal Article
Source:International Journal of Biological Macromolecules, Elsevier, Volume 74, p.318-326 (2015)
Keywords:adipocyte, adipose derived stem cell, adipose tissue, alginate o carboxymethyl chitosan, article, biocompatibility, biodegradability, cell adhesion, cell differentiation, cell proliferation, cellular distribution, chemical parameters, chitosan, complex formation, Compressive strength, controlled study, cross linking, extracellular matrix, hydrogel, hydrogen bond, nano fibrin composite, nanocomposite, Swelling ratio, tissue engineering, Tissue regeneration, tissue scaffold, unclassified drug, Water content, Young modulus
Injectable, biodegradable scaffolds are required for soft tissue reconstruction owing to its minimally invasive approach. Such a scaffold can mimic the native extracellular matrix (ECM), provide uniform distribution of cells and overcome limitations like donor site morbidity, volume loss, etc. So, here we report two classes of biocompatible and biodegradable hydrogel blend systems namely, Alginate/. O-carboxymethyl chitosan (O-CMC) and Alginate/poly (vinyl alcohol) (PVA) with the inclusion of fibrin nanoparticles in each. The hydrogels were prepared by ionic cross-linking method. The developed hydrogels were compared in terms of its swelling ratio, degradation profile, compressive strength and elastic moduli. From these preliminary findings, it was concluded that Alginate/. O-CMC formed a better blend for tissue engineering applications. The potential of the formed hydrogel as an injectable scaffold was revealed by the survival of adipose derived stem cells (ADSCs) on the scaffold by its adhesion, proliferation and differentiation into adipocytes. Cell differentiation studies of fibrin incorporated hydrogel scaffolds showed better differentiation was confirmed by Oil Red O staining technique. These injectable gels have potential in soft tissue regeneration. © 2014 Elsevier B.V.
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