Cell adhesion is an important part of many complex biological processes. It plays crucial roles in cancer, development and maintenance of stem cell compartment. The measurement of adhesion under experimental conditions might provide important information for cell biology. There are several protocols to measure adhesion, usually based on washing or shaking to remove non-adherent cells. Here, we describe a quantification method based on gravitational force to measure adhesion in a 96-well format. Non-adherent cells are separated and only vital cells are quantified with a colorimetric assay. This assay can be used especially when the "anti-adhesion" effect is present only for a short period of time like is the case of peptides or cytokines since it provides a trap for non-adherent cells in a way that they can not touch again the adherent surface. As examples we provide the quantification of cell-cell interaction with blocking antibodies anti-CD44 in hematopoietic stem cells and the effect of the stromal cell derived factor-1 (SDF-1) in the Jurkat cell line when they are in contact with mesenchymal stromal cells. This method facilitates fast and reliable measurement of cell adhesion in multiwell format for screening assays. © 2011 Landes Bioscience.
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Aa Zepeda-Moreno, Taubert, Ia, Hellwig, Ia, Hoang, Va, Pietsch, La, Lakshmanan, V. Kc, Wagner, Wab, and Ho, A. Da, “Innovative method for quantification of cell-cell adhesion in 96-well plates”, Cell Adhesion and Migration, vol. 5, 2011.