An attempt was made to find the optimal proportion of econazole to betamethasone which gives the least inhibition of antifungal activity of econazole. There was a gradual reduction in O2 consumption by Candida tropicalis (ATCC 13803) with increase in the concentration of econazole. Betamethasone stimulated the O2 consumption by C. tropicalis. The changes elicited by betamethasone in various concentrations on the antifungal activity of econazole were investigated by the Warburg manometric technique. There was no significant decrease in antifungal activity of econazole when combined with betamethasone in a 1:0.1 prooortion. The inhibition of O2 consumption was more at a 1:0.1 ratio than at 1:0.125. The minimal inhibitory concentration of econazole was found to be 0.06 .mu.g by plate assay method. It was observed by plate assay and by turbidometric assay that 1:0.075 was the optimal ratio of econazole to betamethasone, which did not give significant decrease in antifungal activity.
K. Shah, RAO, S., Dr. Bipin G. Nair, and MODI, V. V., “Modification of Antifungal Activity of Econazole in Presence of Betamethazone”, INDIAN JOURNAL OF MEDICAL RESEARCH, vol. 73, pp. 965–969, 1981.