Electrochemical sensor for the determination of Vitamin B 12 was fabricated using a disposable pencil graphite electrode. The main highlight of this work is that without any electrode modification and analyte pre-concentration, direct electrochemical detection of Vitamin B 12 was made possible. Morphological and structural studies were incorporated. Electrochemical studies were done using cyclic voltammetry and differential pulse voltammetry. Electrochemical reduction of Vitamin B 12 was observed at -0.82 V. Sensor exhibited a linear range of 2.5-30 nM with a sensitivity of 1.96 µA/nM/cm2. The viability of the sensor towards practical use was tested in commercial Vitamin B 12 tablet.
R. G. Krishnan, Greeshma, S., D. Morris, S., Rameshan, S. S., and Dr. Beena S., “Morphological Studies of Disposable Graphite and its Effective utilization for Vitamin B12 Analysis in Pharmaceutical Formulations”, Materials Today: Proceedings, vol. 18, Part 7, pp. 3314 - 3320, 2019.