A new facile and sensitive spectrophotometric determination of Pindolol (PDL), a beta blocker drug has been developed and validated. The method was based on the reaction between pindolol and K 3 [Fe(CN) 6] in presence of FeCl 3 to form Prussian blue. The absorbance values were recorded at 700 nm and a calibrated graph was constructed. A dynamic Beer's law range was observed in the range 0.125-2.5μgmL -1 with a detection limit of 0.03μgmL -1 and a quantitation limit of 0.08μgmL -1. Various experimental parameters such as effect of solvents, stability, interference effects due to excipients etc were studied. The reproducibility of this methods were checked by six replicate determinations at 1.0μgml -1 PDL and the standard deviation was found to be between 0.20 and 0.42%. The results were statistically compared with those of the reference/literature method by applying Student's t-test and F-test. The sensitivity, simplicity, temperature independence and stability of the colored product are the advantages of the proposed method and it is also free from extraction steps and use of carcinogenic solvents. © 2011 American Institute of Physics.
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Pa Nagaraja, Kumar, H. R. Aa, Bhaskara, B. Lb, and Kumar, S. Ab, “Novel spectrophotometric method for the determination of pindolol in pharmaceutical samples”, in AIP Conference Proceedings, Calicut, Kerala, 2011, vol. 1391, pp. 369-371.