Paclitaxel loaded fibrinogen coated CDTE/ZNTE core shell nanoparticles for targeted imaging and drug delivery to breast cancer cells
Publication Type:Journal Article
Source:Journal of Biomedical Nanotechnology, American Scientific Publishers, Volume 9, Number 10, p.1657-1671 (2013)
Keywords:animal cell, animal experiment, Aqueous-phase synthesis, article, breast cancer, Cadmium telluride, cadnium telluride, calcium chloride, cancer cell, cancer localization, Cells, Cellular imaging, controlled study, Cytology, Diseases, Drug delivery, drug delivery system, drug release, female, fibrinogen, fluorescence microscopy, Glycoproteins, HeLa cell line, human, human cell, hydrogen bond, in vitro study, Integrins, MCF 7 cell line, Medical imaging, mouse, MTT assay, nonhuman, paclitaxel, particle size, QDs, quantum dot, Semiconductor quantum dots, synthesis, tellurium, unclassified drug, very late activation antigen 5, zeta potential, zinc telluride
The study aims at the targeted imaging using CdTe/ZnTe core shell QDs and delivery of paclitaxel (PTX) loaded fibrinogen coated yellow-QDs (PTX-fib-yellow-QDs) towards breast cancer cells via the α5 β1 -integrins. We developed fibrinogen coated different sized CdTe/ZnTe core shell quantum dots of 2-10 nm size, which have been prepared by one-pot aqueous-phase approach. The fib-coated-QDs (fib-coated-QDs) and PTX-fib-yellow-QDs were prepared by twostep coacervation technique using CaCl2 as cross-linker. Particle size of fib-coated-QDs was in between 60-220 nm while PTX-fib-yellow-QDs showed 180 ± 40 nm. The MTT assay confirmed cytocompatibility of fib-coated-QDs on L929 and MCF-7 than bare QDs, whereas significant toxicity toward MCF-7 by PTX-fib-yellow-QDs was observed. The hemocompatible fib-coated-QDs showed enhanced localization and retention toward α5 β1 -integrins +ve MCF-7 compared to α5 β1 -integrins -ve L929 cells. The specific binding of fib-coated-yellow-QDs was further confirmed with α5 β1 -integrins +ve HeLa and α5 β1 -integrins -ve HT29 cells. Cellular uptake studies revealed localization of PTX-fib-coated-yellow-QDs inside MCF-7 cells compared to the normal L929 cells. These results indicated that fib-coated-QDs could be used for targeted imaging and as a suitable "nanocarrier" aiming breast cancer cells. Copyright © 2013 American Scientific Publishers All rights reserved.
cited By 10
Cite this Research Publication
Related Research Publications
- Thermo-responsive fibrinogen nanogels: a viable thermo-responsive drug delivery agent for breast cancer therapy?
- Dual drug encapsulated thermo-sensitive fibrinogen-graft-poly (N-isopropyl acrylamide) nanogels for breast cancer therapy
- 5-Fluorouracil loaded fibrinogen nanoparticles for cancer drug delivery applications
- Multifunctional chitin nanogels for simultaneous drug delivery, bioimaging, and biosensing.
- Folate conjugated carboxymethyl chitosan-manganese doped zinc sulphide nanoparticles for targeted drug delivery and imaging of cancer cells