Publication Type:

Journal Article


Journal of Biomaterials Science, Polymer Edition, Volume 25, Number 4, p.325-340 (2014)



Acetates, Acetic acid, acetic acid ethyl ester, article, Biocompatible Materials, biodegradability, Bone, Bone and Bones, bone tissue, Bone tissue engineering, cell adhesion, Cell culture, cell differentiation, cell interaction, cell proliferation, cell spreading, Composite scaffolds, conductance, Contact angle, controlled study, degradation, electric conductivity, Electrospinning, Favorable interactions, Fourier transform infrared photoacoustic spectroscopy, gelatin, human, human cell, Human mesenchymal stem cells (hMSCs), Humans, hydrophilicity, Hydrophobic and Hydrophilic Interactions, Materials Testing, mesenchymal stem cell, Mesenchymal Stromal Cells, Mixtures, nanocomposite, nanofiber, Nanofibers, Nanofibrous scaffolds, PCL, pH, polycaprolactone, Polyesters, priority journal, Scaffolds (biology), Solutions, Solvents, Stem cells, tissue engineering, Tissue Scaffolds, viscosity


<p>Composite nanofibrous scaffolds with various poly(ε-caprolactone) (PCL)/gelatin ratios (90:10, 80:20, 70:30, 60:40, 50:50 wt.%) were successfully electrospun using diluted acetic and ethyl acetate mixture. The effects of this solvent system on the solution properties of the composites and its electrospinning properties were investigated. Viscosity and conductivity of the solutions, with the addition of gelatin, allowed for the electrospinning of uniform nanofibers with increasing hydrophilicity and degradation. Composite nanofibers containing 30 and 40 wt.% gelatin showed an optimum combination of hydrophilicity and degradability and also maintained the structural integrity of the scaffold. Human mesenchymal stem cells (hMSCs) showed favorable interaction with and proliferation on, the composite scaffolds. hMSC proliferation was highest in the 30 and 40 wt.% gelatin containing composites. Our experimental data suggested that PCL-gelatin composite nanofibers containing 30-40 wt.% of gelatin and electrospun in diluted acetic acid-ethyl acetate mixture produced nanofiber scaffolds with optimum hydrophilicity, degradability, and bio-functionality for stem cell-based bone tissue engineering. © 2013 Taylor &amp; Francis.</p>


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Cite this Research Publication

N. Sa Binulal, Natarajan, Aa, Menon, Da, Bhaskaran, V. Kb, Mony, Ua, and Nair, S. Va, “PCL-gelatin composite nanofibers electrospun using diluted acetic acid-ethyl acetate solvent system for stem cell-based bone tissue engineering”, Journal of Biomaterials Science, Polymer Edition, vol. 25, pp. 325-340, 2014.