Publication Type:

Journal Article


International Journal of Biological Macromolecules, Volume 58, p.220-224 (2013)



animal cell, Animals, article, Blood Cells, blood outgrowth endothelial cell, cell adhesion, Cell Culture Techniques, cell function, cell proliferation, cell shape, cell structure, Cell Survival, cell type, cell viability, Cells, chitosan, collagen, controlled study, Culture Media, Cultured, Endothelial cells, endothelium cell, Fourier Transform Infrared, Lactic acid, molecular scaffold, nanocomposite, Nanofibers, Nitric Oxide Synthase Type III, nonhuman, polylactic acid, Polymers, rat, Rats, Regenerative medicine, Spectroscopy, Tissue Scaffolds


In this work, the attachment, viability and functionality of rat Blood Outgrowth Endothelial Cells (rBOEC) and genetically modified rBOEC (rBOEC/eNOS-GFP), which over express endothelial nitric oxide synthase (eNOS), were investigated on Poly(lactic acid) (PLA)-chitosan and PLA-chitosan-collagen nanofibrous scaffolds. Both the cell types displayed good attachment, remained viable and functional on both scaffolds. Moreover, incorporation of collagen in the scaffold helped in sustaining the rBOEC for upto one week, although collagen was not found necessary for rBOEC/eNOS-GFP. We conclude that PLA-chitosan based nanofibrous scaffolds can be a potential candidate for BOEC based wound healing applications. © 2013 Elsevier B.V.


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Cite this Research Publication

, “Poly (lactic acid)-chitosan-collagen composite nanofibers as substrates for blood outgrowth endothelial cells”, International Journal of Biological Macromolecules, vol. 58, pp. 220-224, 2013.