Publication Type:

Journal Article


International Journal of Biological Macromolecules, Volume 44, Number 1, p.1-5 (2009)



Apatite, article, Artificial, beta chitin, biocompatibility, calcium chloride, cell adhesion, cell spreading, Cephalopoda, chitin, composite material, controlled study, disodium hydrogen phosphate, Durapatite, Electron, Fourier Transform Infrared, human, human cell, Hydroxyapatite, infrared spectroscopy, Materials Testing, Membranes, mesenchymal stem cell, Mesenchymal Stem Cells, microscopy, Scanning, scanning electron microscopy, Spectroscopy, tissue engineering, unclassified drug, X ray powder diffraction, X-Ray Diffraction


β-Chitin is a biopolymer principally found in shells of squid pen. It has the properties of biodegradability, biocompatibility, chemical inertness, wound healing, antibacterial and anti-inflammatory activities. Hydroxyapatite (HAp) is a natural inorganic component of bone and teeth and has osteoconductive property. In this work, β-chitin-HAp composite membranes were prepared by alternate soaking of β-chitin membranes in CaCl 2 (pH 7.4) and Na 2HPO 4 solutions for 2 h in each solution. After 1, 3 and 5 cycles of immersion, β-chitin membranes were characterized using the SEM, FT-IR, EDS and XRD analyses. The results showed the presence of apatite layer on surface of β-chitin membranes, and the amounts of size and deposition of apatite layers were increased with increasing number of immersion cycles. Human mesenchymal stem cells (hMSCs) were used for evaluation of the biocompatibility of pristine as well as composite membranes for tissue engineering applications. The presence of apatite layers on the surface of β-chitin membranes increased the cell attachment and spreading suggesting that β-chitin-HAp composite membranes can be used for tissue engineering applications.


cited By (since 1996)42

Cite this Research Publication

K. Madhumathi, Binulal, N. S., Nagahama, H., Tamura, H., Shalumon, K. T., Selvamurugan, N., Nair, S. V., and Dr. Jayakumar Rangasamy, “Preparation and characterization of novel β-chitin-hydroxyapatite composite membranes for tissue engineering applications”, International Journal of Biological Macromolecules, vol. 44, pp. 1-5, 2009.