Electrospun nano, micro and micro/nano (multiscale) poly(caprolactone) (PCL) fibrous scaffolds with and without nano hydroxyapatite (nHAp) was prepared. All the scaffolds were evaluated for its spectroscopic, morphological, mechanical, thermal, cell attachment and protein adsorption properties. The cell attachment studies showed that cell activity on the nano-fibrous, as well as multi-scale scaffolds with and without nHAp was higher compared to micro-fibrous scaffolds. A time dependent cell attachment study on aligned micro-fibers was carried out to elucidate the difference in cell interaction on micro-fibers. The cell activity, proliferation and total protein adsorption on the nano-fibers/nano-fibers with nHAp was significantly higher than on the micro-fibers, although the adsorption per unit area was less on the nano-fibers due to the much higher surface area of nano-fibers. These results suggest that a combination of a micro- and nano-fiber hierarchical scaffold could be more beneficial for tissue engineering applications than the individual scaffolds provided the amount of nano- fibers could be suitably optimized. Copyright © Taylor & Francis Group, LLC.
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K. T. Shalumon, Binulal, N. S., Deepthy, M., Dr. Jayakumar Rangasamy, Manzoor, K., and Nair, S. V., “Preparation, characterization and cell attachment studies of electrospun multi-scale poly(caprolactone) fibrous scaffolds for tissue engineering”, Journal of Macromolecular Science, Part A: Pure and Applied Chemistry, vol. 48, pp. 21-30, 2011.